Application of Time of Flight Mass Spectrometry in the Identification of Dendrobium devonianum Paxt and Dendrobium officinale Kimura et Migo Grown in Longling Area of Yunnan, China
文献类型： 外文期刊
作者： Lin, Tao 1 ; Chen, Xing-Lian 1 ; Wang, Jing 2 ; Hu, Zheng-Xu 2 ; Wu, Guang-Wei 3 ; Sha, Ling-Jie 1 ; Cheng, Long 4 ; Liu, Hong-Cheng 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Yunnan, Peoples R China
2.Longling Agr Environm Protect Monitoring Stn, Baoshan 678300, Peoples R China
3.Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Peoples R China
4.SCIEX Analyt Instrument Trading Co Ltd, Shanghai 200335, Peoples R China
关键词： Time of flight mass spectrometry; identification; Dendrobium devonianum; Longling area
期刊名称：SEPARATIONS （ 影响因子：3.344； 五年影响因子：2.943 ）
ISSN：
年卷期： 2022 年 9 卷 5 期
页码：
收录情况： SCI
摘要： In this study, in order to protect the characteristic Dendrobium devonianum Paxt industry in the Longling area, and promote the healthy development of its characteristic Chinese herbal medicines in Yunnan Province, China, the identification of Dendrobium devonianum Paxt and Dendrobium officinale Kimura et Migo from Longling county was discussed using time of flight mass spectrometry. The data of 13 Dendrobium devonianum and 7 Dendrobium officinale in the Longling area were collected by TOF MS-IDA-15 MS/MS mode, and the collected data were analyzed by PCA and T-test using MarkerView software, and the difference markers were searched using the database to confirm their compound structures. In positive and negative ion modes, 3645 and 2344 peaks were detected, respectively; 64 positive ion compounds and 60 negative ion compounds, for a total of 124 compounds were identified, mainly including organic acids, polyphenols, alkaloids, amino acids and their derivatives, benzene and its derivatives, and other compounds. The score plot and loading plot analyzed by PCA show that Dendrobium devonianum and Dendrobium officinale collected in the Longling area can be effectively identified and differentiated by high-resolution mass spectrometry with the 15 different markers in positive ion mode and 17 markers in negative ion mode, respectively. The successful identification of Dendrobium devonianum and Dendrobium officinale fully demonstrates that TOF MS can be effectively used in the identification of Dendrobium and related Chinese herbal medicines with broadly application foreground.
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