Rapid Identification of Alien Chromosome Fragments and Tracing of Bioactive Compound Genes in Intergeneric Hybrid Offspring Between Brassica napus and Isatis indigotica Based on AMAC Method
文献类型： 外文期刊
作者： Guo, Yanhong 1 ; Han, Yutian 2 ; Gao, Jinxiang 2 ; Ge, Xianhong 4 ; Luo, Yanqing 2 ; Zhao, Kaiqin 2 ; Li, Genze 2 ; Zu, Feng 2 ; Cheng, Xiaomao 3 ;
作者机构： 1.Yunnan Univ, Sch Agr, Kunming 650504, Peoples R China
2.Yunnan Acad Agr Sci, Ind Crops Res Inst, Yunnan Key Lab Genet Improvement Herbal Oil Crops, Kunming 650225, Peoples R China
3.Southwest Forestry Univ, Coll Landscape Architecture & Hort Sci, Engn Technol Res Ctr, Natl Forestry & Grassland Adm Southwest Landscape, Kunming 650224, Peoples R China
4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
关键词：
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 5 期
页码：
收录情况： SCI
摘要： Distant hybridization between Brassica napus and related genera serves as an effective approach for rapeseed germplasm innovation. Isatis indigotica, a wild relative of Brassica, has emerged as a valuable genetic resource for rapeseed improvement due to its medicinal properties. This study employed anchor mapping of alien chromosomal fragment localization (AMAC) method to efficiently identify alien chromosomal fragments in the progeny derived from distant hybridization between I. indigotica and Brassica napus, 'Songyou No. 1'. Based on the AMAC method, we developed 193,101 IP and SSR markers utilizing the I. indigotica reference genome (Woad-v1.0). Through Electronic-PCR analysis against the Brassica and I. indigotica pan-genome, 27,820 specific single-locus (SSL) IP and SSR markers were obtained. Subsequently, 205 pairs of IP primers and 50 pairs of SSR primers were synthesized randomly, among which 148 pairs of IP markers (72.20%) and 45 pairs of SSR markers (90%) were verified as SSL molecular markers for the I. indigotica genome with no amplification product in four Brassica crops. These 193 SSL markers enable precise identification of one complete I6 chromosome and three chromosomal fragments (I1:1.17 Mb, I5:2.61 Mb, I7:1.11 Mb) in 'Songyou No. 1'. Furthermore, we traced 32 genes involved in bioactive compound biosynthesis within/near these alien segments in 'Songyou No. 1' and developed seven functional markers. This study not only validates the efficacy of SSL markers for detecting exogenous chromatin in intergeneric hybrids but also provides valuable insights for the precise identification and mapping of desired chromosomal fragments or genes embedded in the derivatives from distant hybridization and potential applications in marker-assisted breeding for medicinal plant via distant hybridization strategy between I. indigotica and Brassica crops.
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