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Development of a rapid and efficient system for CR genes identification based on hairy root transformation in Brassicaceae

文献类型： 外文期刊

作者： Yu, Wenlin 1 ; Yang, Lu 1 ; Xiang, Yuanyuan 1 ; Li, Rongde 4 ; Zhou, Xueqing 1 ; Gan, Longcai 1 ; Xiang, Xianyu 1 ; Zhang, Yunyun 5 ; Yuan, Lei 5 ; Luo, Yanqing 5 ; Li, Genze 5 ; Wang, Youning 6 ; Chen, Yinhua 3 ; Chen, Peng 1 ; Zhang, Chunyu 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China

3.Hainan Univ, Sanya Nanfan Res Inst, Natl Key Lab Trop Crop Breeding, Sanya 572025, Hainan, Peoples R China

4.Natl Agrotech Extens & Serv Ctr, Beijing 100125, Peoples R China

5.Yunnan Acad Agr Sci, Ind Crops Res Inst, Kunming 650221, Yunnan, Peoples R China

6.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China

关键词： Brassicaceae; Agrobacterium rhizogenes; Hairy root transformation; Clubroot; Gene function

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.5 ）

ISSN： 2095-9885

年卷期： 2024 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae infection. Breeding of clubroot resistant cultivars based on mapping and cloning of resistant genes is commonly regarded as the most cost-effective and efficient way to fight against this disease. The traditional way of R gene functional validation requires stable transformation that is both time- and labor-consuming. In this study, a rapid and efficient hairy-root transgenic protocol mediated by Agrobacterium rhizogenes was developed. The transformation positive rate was over 80% in Brassica napus showed by GUS reporter gene and this transformation only took 1/6 of the time compared with stable transformation. The system was applicable to different B. napus varieties and other cruciferous crops including Brassica rapa and Brassica oleracea. In particular, two known CR genes, CRA3.7.1 and CRA8.2.4 were used respectively, as example to show that the system works well for CR gene study combined with subsequent P. brassicae infection in B. napus. Most importantly, it works both in over-expression that led to disease resistance, as well as in RNAi which led to disease susceptible phenotype. Therefore, this system can be used in batch-wise identification of CR genes, and also offered the possibility of manipulating key genes within the P. brassicae genome that could improve our knowledge on host-pathogen interaction.

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