文献类型： 外文期刊

作者： Chen Xinglian ¹ ; Lin Tao ¹ ; Liu Xingyong ¹ ; Mei Wenquan ¹ ; Yang Dongshun ¹ ; Li Yangang ¹ ; Li Maoxuan ¹ ; Wang Luxiang ¹ ;

作者机构： 1.Yunnan Acad Agr Sci, Qual Standardizing & Testing Technol Inst, Kunming 650224, Yunnan, Peoples R China; Minist Rural Agr, Supervis & Testing Ctr Farm Prod Qual, Kunming Sect, Kunming 650224, Yunnan, Peoples R China

关键词： dispersive solid phase extraction (DSPE); ultra high performance liquid chromatography (UHPLC); tandem mass spectrometry (MS/MS); antibiotics; triphenylmethanes; fish; shrimp; veterinary drug residue

期刊名称：CHINESE JOURNAL OF CHROMATOGRAPHY （ 影响因子：0.7； 五年影响因子：0.5 ）

ISSN： 1000-8713

年卷期： 2019 年 37 卷 9 期

页码：

收录情况： SCI

摘要： A method was established to rapidly determine 20 kinds of veterinary drug residues including three catagories of antibiotics (sulfonamides, quinolones, and chloramphenicols) and two kinds of triphenylmethanes (malachite green (MG) and leucomalachite green (LMG)) in fish and shrimp, based on dispersive solid phase extraction purification-ultra high performance liquid chromatography-tandem mass spectrometry. The samples were first hydrolyzed using a dipotassium hydrogen phosphate solution, and then extracted using acetonitrile. Afterward, the extraction solution was dehydrated and salted out with sodium chloride and condensed to nearly dry using a rotating evaporator. This residue was dissolved in 1.0 mL methanol. The resulting solution was purified by dispersive solid phase extraction method with C18 and PSA sorbents, and filtered through a filter. The target compounds were separated employing a ZORBAX C18 column. The mass spectrometer datas were acquired by multiple reaction monitoring (MRM) of positive and negative modes and quantitated applying the isotope internal standard method. The 20 veterinary drugs showed a good linear relationship in the range of 0.2-300 mu g/L. The limits of detection and the limits of quantification were 0.1-0.6 and 0.3-1.8 mu g/kg, respectively, while the correlation coefficients were greater than 0.99. The average recoveries at the three spiked levels (1, 5, and 20 times of quantitative limits) ranged between 72.5%-118%, with the relative standard deviations of 1.9%-9.8%. The advantages of method include a simple pretreatment, a high detection efficiency, and a low cost. Moreover, it is suitable for the simultaneous determination of multiple veterinary drug residues in fish and shrimp.