文献类型： 外文期刊

作者： Chen Xinglian ¹ ; Lin Tao ¹ ; Liu Xingyong ¹ ; Mei Wenquan ¹ ; Wang Li ¹ ; Geng Huichun ¹ ; Cheng Long ² ; Wang Luxiang ¹ ;

作者机构： 1.Minist Rural Agr Kunming, Supervis & Testing Ctr Farm Prod Qual, Qual Standardizing & Testing Technol Inst, Yunnan Acad Agr Sci, Kunming 650205, Yunnan, Peoples R China

2.SCIEX Analyt Instrument Trading Co Ltd, Shanghai 200335, Peoples R China

关键词： ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS); dispersive solid phase extraction; simultaneous extraction and purification; prohibited and restricted veterinary drugs; fish; shrimp

期刊名称：CHINESE JOURNAL OF CHROMATOGRAPHY （ 影响因子：0.7； 五年影响因子：0.5 ）

ISSN： 1000-8713

年卷期： 2020 年 38 卷 5 期

页码：

收录情况： SCI

摘要： A method was established for the rapid determination of 28 prohibited and restricted veterinary drugs of five classes ( sulfonamides, quinolones, chloramphenicols, nitrofurans metabolites and triphenylmethane) in fish and shrimp by ultra high performance liquid chromatography-tandem mass spectrometry ( UHPLC-MS/MS) coupled with simultaneous extraction and purification. After optimizing the sample pretreatment methods and chromatographic conditions, the samples were hydrolyzed with hydrochloric acid. The five kinds of residual drugs were simultaneously extracted with acetonitrile after nitrofuran metabolites derivatized with 2-nitrobenzaldehyde. The extraction solutions were dehydrated with sodium chloride and salted out. The acetonitrile layer was purified with n-hexane and concentrated to near dryness. The residues were dissolved in 1. 0 mL methanol. Then, the solutions were purified by dispersive solid phase extraction with C18, N-( n-propyl) ethylenediamine ( PSA) and amino bonded silica gel ( NH2) sorbents. The residues were separated on a ZORBAX C18 chromatographic column ( 50 mmx2. 1 mm, 1. 8 mu m). The MS data were acquired in positive and negative multiple reaction monitoring ( MRM) modes, and quantitated by the isotope internal standard method. With this approach, the linear relationships of the 28 veterinary drugs were good. The linear correlation coefficients were greater than 0. 99. The limits of detection and limits of quantification were in the range of 0. 1- 0. 8 mu g / kg and 0. 3- 2. 7 mu g / kg, respectively. The average recoveries at three spiked levels (1, 5, and 20 fold of the LOQs) were 70. 0% - 120% with relative standard deviations of 0. 9% -10. 0%. The method is simple, rapid, accurate and sensitive, and it is suitable for the rapid determination of various prohibited and restricted drugs in fish and shrimp.