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In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

文献类型： 外文期刊

作者： Yu, Rongpei 1 ; Cheng, Ying 4 ; Pu, Yanfei 4 ; Li, Fan 2 ; Lu, Shugang 1 ;

作者机构： 1.Yunnan Univ, Sch Life Sci, Sch Ecol & Environm Sci, Inst Ecol & Geobot, Kunming 650091, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Natl Engn Res Ctr Ornamental Hort, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China

3.Yuxi Yunxing Biotech Co Ltd, Yuxi 653100, Yunnan, Peoples R China

4.Yunnan Univ, Sch Agr, Kunming 650504, Yunnan, Peoples R China

关键词： cytokinin; in vitro propagation; resurrection plants; Selaginella pulvinata

期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）

ISSN： 0018-5345

年卷期： 2021 年 56 卷 3 期

页码：

收录情况： SCI

摘要： The resurrection plant Selaginella pulvinata (Hook. & Grey.) Maxim is used as an ornamental and medicinal plant. It is also a good candidate for exploring the desiccation tolerance of resurrection plants. However, there is not an efficient propagation method for S. pulvinata. In the present study, we evaluated the establishment of in vitro propagation of S. pulvinata using frond tips as explants. The original shoot induction, adventitious shoot proliferation and plantlet growth media, and substrate type of plantlet acclimatization were investigated. The highest induction rate of original shoots (61.77 +/- 5.17%) was obtained on half-strength (1/2) MS medium supplemented with 0.1 mg.L-1 N-6-benzylaminopurine (BAP). The 1/2 MS with 1.0 mg.L-1 BAP was the most effective medium for the adventitious shoot proliferation. The quarter-strength (1/4) MS containing 0.1% (w/v) active charcoal (AC) was optimum for plantlets proliferated from adventitious shoots and plantlet growth. Approximately 98 plantlets could be obtained from one single original shoot via one-time shoot proliferation cultivation and plantlet cultivation. The acclimated plants on a 5:1 (v/v) mixture of peat and perlite had the highest survival rate (92.13 +/- 1.67%). The acclimated plants maintained excellent resurrection ability.

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