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Construction of a genome-wide SSR marker library in Gerbera hybrida: Insights into genetic variation and germplasm resources

文献类型： 外文期刊

作者： Yuan, Huijuan 1 ; Zhou, Qian 1 ; Wani, Muneeb Ahmad 4 ; Yang, Shaohua 2 ; Lu, Zhenhong 1 ; Shan, Qili 1 ; Li, Shenchong 1 ; Li, Fan 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Floriculture Res Inst, Natl Engn Res Ctr Ornamental Hort, Key Lab Flower Breeding Yunnan Prov, Kunming, Peoples R China

2.Yunnan Acad Agr Sci, Inst Alpine Econ & Bot, Lijiang, Peoples R China

3.Yunnan Univ, Sch Agr, Kunming 650504, Peoples R China

4.Sher Ekashmir Univ Agr Sci & Technol Kashmir, Fac Hort, Dept Floriculture & Landscape Architecture, Srinagar 190025, India

关键词： Gerbera hybrida; SSR markers; Genetic analysis; Fingerprint library; Germplasm identification

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）

ISSN： 0304-4238

年卷期： 2024 年 324 卷

页码：

收录情况： SCI

摘要： Gerbera hybrida is a highly sought-after cut flower globally, renowned for its diverse flower color and pattern resulting from its complex inflorescence meristem architecture. However, the scarcity of genomic information and molecular markers has impeded efforts to comprehend the genetic diversity of G. hybrida. To address this, our study aimed to construct genome-wide simple sequence repeat (SSR) markers in G. hybrida, utilizing genomic data from an inbred line. We employed 170 gerbera cultivars exhibiting distinct flower patterns and colors to analyze genetic variation and diversity. Our analyses revealed highly polymorphic SSR markers, a total of 69 alleles (Na) were amplified by 14 pairs of primers, in which polymorphic information content (PIC) ranging from 0.380 to 0.700 with an average value of 0.583. The polymorphic results of the observed heterozygosity (Ho) and Shannon's information index (I) were 0.499 and 1.195, respectively, showing a high level of genetic diversity within the enriched sequences of the G. hybrida genome. Leveraging these markers with strong differentiation, we successfully constructed a unique fingerprint map encompassing 170 gerbera germplasm lines. This study involved the construction of an SSR fingerprint library for G. hybrida germplasm resources, employing genetic analysis for screening and molecular-level identification. The findings provide valuable insights into the identification of gerbera germplasm resources and kinship studies among various groups. Moreover, this study lays a solid foundation for future advancements in molecular design breeding improvement and germplasm resource development in G. hybrida.

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