Natural surfactant used as an additive in the homogenate-ultrasound-synergistic extraction of regaloside A and polysaccharides from Lilium lancifolium bulbs using Doehlert matrix design
文献类型： 外文期刊
作者： Zhang, Aoqi 1 ; Zhao, Ru 1 ; Wang, Yunxia 1 ; Liu, Tingting 3 ; Tian, Hao 4 ; Yin, Xing 1 ; Yang, Lei 1 ; Sui, Xiaoyu 3 ;
作者机构： 1.Northeast Forestry Univ, Coll Chem Chem Engn & Resource Utilizat, Harbin 150040, Peoples R China
2.Nanjing Xiaozhuang Univ, Key Lab Qual & Safety Agr Prod Nanjing, Nanjing 211171, Peoples R China
3.Qiqihar Med Univ, Coll Pharm, Qiqihar 161006, Peoples R China
4.Yunnan Acad Agr Sci, Inst Agroprod Proc, Kunming 650223, Yunnan, Peoples R China
5.Heilongjiang Prov Key Lab Ecol Utilizat Forestry, Harbin 150040, Peoples R China
6.Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China
关键词： Lilium lancifolium; Homogenate-ultrasound-synergistic extraction; Regaloside A; Polysaccharides; n-hexyl glucoside; Natural surfactant
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.449； 五年影响因子：6.508 ）
ISSN： 0926-6690
年卷期： 2022 年 188 卷
页码：
收录情况： SCI
摘要： In this study, 10 different natural surfactants were compared as additives for the simultaneous extraction of lipophilic regaloside A and water-soluble polysaccharides from tiger lily (Lilium lancifolium Thunb.) bulbs by homogenate-ultrasound-synergistic extraction. Four significant factors that affect the yields of regaloside A and polysaccharides, including homogenate speed, ultrasound irradiation power, reaction temperature and ultrasound irradiation time, were screened by single-factor experiments and Plackett-Burman design and then optimized using Doehlert matrix design combined with a desirability function. The optimal extraction process conditions were obtained as follows: APG06 (n-hexyl glucoside) surfactant concentration of 0.6%, homogenate speed of 20000 r/min, homogenate time of 6 min, liquid-solid ratio of 20 mL/g, ultrasound irradiation power of 300 W, ultrasound irradiation time of 52 min, reaction temperature of 62.8 degrees C, and buffer and cavitation times of 1.5 s and 2.0 s, respectively. Under the optimal extraction conditions, regaloside A and polysaccharides yields of 2.797 +/- 0.084 mg/g and 0.493 +/- 0.016 g/g are obtained with relative standard deviations of 3.00% and 3.50%, respectively. The results of the method validation and comparison with the conventional method show that homogenate-ultrasound-synergistic extraction with APG06 as an additive is a green, efficient and energy-saving extraction method. Compared with the traditional extraction method, HUSE with APG06 as an additive is an effective method to extract regaloside A and polysaccharides from L. lancifolium bulbs. This study provides a reference for the development of green low carbon natural product extraction process.
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