文献类型： 外文期刊
作者： Zhang, Rong-Yue 1 ; Li, Wen-Feng 1 ; Huang, Ying-Kun 1 ; Wang, Xiao-Yan 1 ; Shan, Hong-Li 1 ; Li, Jie 1 ; Cang, Xiao-Y 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Sugarcane Res Inst, Yunnan Key Lab Sugarcane Genet Improvement, Kaiyuan 661699, Peoples R China
关键词： Yunnan province; Puer; Phytoplasma; Sugarcane white leaf; 16SrXI-B
期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）
ISSN： 0972-1525
年卷期： 2019 年 21 卷 5 期
页码：
收录情况： SCI
摘要： To confirm the phytoplasma association in 13 sugarcane white leaf (SCWL) symptomatic samples collected from Puer, China, nested PCR assays with phytoplasma universal primer pairs was performed. A similar to 1240 bp product was obtained from 10 out of 13 symptomatic samples, and 16S rRNA gene sequencing results showed that all sequences of SCWL isolates were 100% identical to each other. BLASTn analysis revealed 100% sequence similarity with the 16S rRNA genes from SCWL phytoplasma strains LC7 and LC9 from Lincang, Yunnan province, China belonging to 16SrXI-B subgroup. Virtual restriction fragment length polymorphism also confirmed that Puer SCWL strains were member of the 16SrXI-B subgroup.
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