文献类型： 外文期刊

作者： Wang, Pingyang ¹ ; Zhao, Qiaoling ¹ ; Qiu, Zhiyong ¹ ; Bi, Simin ¹ ; Wang, Wenbo ¹ ; Wu, Meina ¹ ; Chen, Anli ⁴ ; Xia, Di ¹ ;

作者机构： 1.Jiangsu Univ Sci & Technol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Sch Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Sericulture Res Inst, Zhenjiang 212018, Jiangsu, Peoples R China

3.Guangxi Zhuang Autonomous Reg Res Acad Sericultur, Nanning 530007, Guangxi, Peoples R China

4.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi 661101, Yunnan, Peoples R China

关键词： Quail-like mutant; Orcokinin; Pigmentation; Neuropeptide; Bombyx mori

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2019 年 114 卷

页码：

收录情况： SCI

摘要： The natural colorful cuticles of insects play important roles in many physiological processes. Pigmentation is a physiological process with a complex regulatory network whose regulatory mechanism remains unclear. Bombyx mori pigmentation mutants are ideal materials for research on pigmentation mechanisms. The purple quail-like (q-l(p)) and brown quail-like (q-l(b)) mutants originated from plain silkworm breeds 932VR and 0223JH respectively exhibit similar cuticle pigmentation to that of the quail mutant. The q-l(p) mutant also presents a developmental abnormality. In this study, genes controlling q-l(p) and q-l(b) mutants were located on chromosome 8 by positional cloning. Then the neuropeptide gene orcokinin (OK) was identified to be the major gene responsible for two quail-like mutants. The B. mori orcokinin gene (BommoOK) produces two transcripts, BommoOKA and BommoOKB, by alternative splicing. The CRISPR/Cas9 system and orcokinin peptides injection were used for further functional verification. We show a novel function of BommoOKA in inhibiting pigmentation, and one mature peptide of orcokinin A, OKA_type2, is the key factor in pigmentation inhibition. These results provide a reference for studying the function of orcokinin and are of theoretical importance for studying the regulatory mechanism of pigmentation.