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The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori

文献类型: 外文期刊

作者: Chen, Anli 1 ; Liao, Pengfei 1 ; Li, Qiongyan 1 ; Zhao, Qiaoling 2 ; Yang, Weike 1 ; Zhu, Shuifen 1 ; Wu, Fang 2 ; He, Ro 1 ;

作者机构: 1.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi, Yunnan, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang, Jiangsu, Peoples R China

期刊名称:PLOS ONE ( 影响因子:3.24; 五年影响因子:3.788 )

ISSN: 1932-6203

年卷期: 2015 年 10 卷 6 期

页码:

收录情况: SCI

摘要: Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B. mori. This mutant features red-colored eggs and embryonic lethality. Genetic analysis showed that Fuyin-lre follows recessive inheritance, with the red egg gene re governing the egg color, and the embryonic lethality of Fuyin-lre may be caused by mutations of other genes closely linked to re. Digital gene expression (DGE) was employed to compare the transcription profiles of Fuyin and Fuyin-lre eggs after 24 and 48 h of incubation. A total of 48 differentially expressed genes followed the same expression patterns in both groups at both time points (FDR < 0.01 and log 2 Ratio >= 1). Further analyses indicated that 8 out of the 48 genes (including re) were closely linked to re. These 8 genes were highly expressed in wild-type Fuyin and the red egg mutant re but showed nearly absent expression in Fuyin-lre. Sequencing of the re gene confirmed that the re gene itself does not induce embryonic lethality, and structure analysis showed that the structural variation of the region where the 8 genes were located may be associated with the embryonic lethality of Fuyin-lre. The present work provides a good foundation for future studies on the mechanism of embryonic lethality and embryonic development in Fuyin-lre.

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