Screening of Polymorphic SSR Molecular Markers Between Resistant and Susceptible Parents for Localization of Brown Rust Resistance Gene
文献类型： 外文期刊
作者： Shan, Hongli 1 ; Li, Wenfeng 1 ; Huang, Yingkun 1 ; Wang, Xiaoyan 1 ; Zhang, Rongyue 1 ; Li, Jie 1 ; Luo, Zhiming 1 ; Yin 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Sugarcane Res Inst, Yunnan Key Lab Sugarcane Genet Improvement, Kaiyuan 661699, Yunnan, Peoples R China
关键词： Sugarcane; Brown rust; Resistance gene; Parent; SSR markers
期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）
ISSN： 0972-1525
年卷期： 2020 年 22 卷 1 期
页码：
收录情况： SCI
摘要： Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Exploring novel resistance genes and breeding varieties with durable resistance is the most economical and effective way of controlling brown rust. The establishment of complete and high-density molecular genetics maps is a key prerequisite for the discovery and location of sugarcane brown rust resistance genes. To obtain more polymorphic simple sequence repeat (SSR) markers for the construction of genetic linkage maps, six highly resistant varieties and six highly susceptible varieties were used as parents to screen the primers with clear bands, obvious polymorphism, and good repeatability. The highest numbers of polymorphic primers were obtained from the parental group of Liucheng 03-1137 x Dezhe 93-88 (52.38%). Ten pairs of primers including mSSCIR34 exhibited high polymorphism in the parental group of Liucheng 03-1137 x Dezhe 93-88. Here, we recommend ten SSR pairs of polymorphic primers including mSSCIR34 to use in the mapping populations of Liucheng 03-1137 x Dezhe 93-88. The polymorphic primers could facilitate the construction of genetic maps and lay an appropriate foundation for the localization of brown rust resistance genes and development of molecular genetics linkage markers.
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