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A multiple reverse transcription PCR assay for simultaneous detection of four main viruses in kiwifruit

文献类型： 外文期刊

作者： Peng, Qiding 1 ; Qiu, Long 1 ; Yang, Ting 1 ; Ning, Jiachen 1 ; Xu, Qianyi 1 ; Dong, Jiahong 2 ; Xi, Dehui 1 ;

作者机构： 1.Sichuan Univ, Key Lab Bioresource & Ecoenvironm, Minist Educ, Coll Life Sci, Chengdu 610065, Sichuan, Peoples R China

2.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Yunnan Prov Key Lab Agr Biotechnol,Minist Agr, Key Lab Southwestern Crop Gene Resource & Germpla, Kunming 650204, Yunnan, Peoples R China

关键词： Kiwifruit; Viruses; Multiplex RT-PCR; Detection

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2020 年 156 卷 4 期

页码：

收录情况： SCI

摘要： Kiwifruit (Actinidia spp.) is an important horticultural crop in the world. Previous studies showed that a number of viruses were found in kiwifruit around the world. To detect the multiple viruses in kiwifruit quickly and effectively, a multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed to detect four viruses simultaneously in kiwifruit: Actinidia chlorotic ringspot-associated virus (AcCRaV), Actinidia virus 1(AcV-1), Actinidia virus A (AcVA), Citrus leaf blotch virus (CLBV), respectively. Four pairs of primers were designed according to the coat protein (CP) genes of the aforementioned viruses and then were confirmed by RT-PCR assay. Actin gene (ACT1) was used as an internal control to improve reliability of the multiplex RT-PCR. Determination of detection limit of the multiplex RT-PCR showed that this method could detect four viruses at the dilution of 10(-4) cDNA. Furthermore, the developed multiplex RT-PCR could detect viruses in kiwifruit field samples successfully.

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