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Identification and Expression Pattern Analysis of Bacterial Blight Resistance Genes in Oryza officinalis Wall ex Watt Under Xanthomonas oryzae Pv. oryzae Stress

文献类型： 外文期刊

作者： Jiang, Chunmiao 1 ; Xiao, Suqin 1 ; Li, Dingqin 4 ; Chen, Ling 1 ; Zhong, Qiaofang 1 ; Yin, Fuyou 1 ; Yu, Tengqiong 1 ; K 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming, Yunnan, Peoples R China

2.Jiangxi Sci & Technol Normal Univ, Jiangxi Key Lab Bioproc Engn, Coll Life Sci, Nanchang, Jiangxi, Peoples R China

3.Jiangxi Sci & Technol Normal Univ, Coinnovat Ctr In Vitro Diagnost Reagents & Device, Coll Life Sci, Nanchang, Jiangxi, Peoples R China

4.Southwest Med Univ, Sch Basic Med Sci, Luzhou, Sichuan, Peoples R China

5.Yunnan Acad Agr Sci, Food Crop Inst, Kunming, Yunnan, Peoples R China

关键词： Oryza officinalis Wall ex Watt; Bacterial blight; Leaf microstructure; Resistance genes; Gene expression

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN： 0735-9640

年卷期： 2019 年 37 卷 5-6 期

页码：

收录情况： SCI

摘要： Background Bacterial blight (BB) caused by Xanthomonas oryzae Pv. oryzae (Xoo) is one of the most serious diseases of rice worldwide. Oryza officinalis Wall ex Watt, harboring abundant genetic diversity and disease resistance features, are important resources of exploring resistance genes with broad-spectrum resistance to BB. However, the molecular mechanisms and genes of BB resistance in O. officinalis have been rarely explored. Results Here, the BB resistance of four different origin O. officinalis populations in Yunnan were identified by seven representative hypervirulent Xoo races, which exhibited different BB resistance among four populations, in which the BB resistance of the Gengma_Lincang population was the strongest. In addition, the pathogenetic ability of seven Xoo races to O. officinalis was different in that the pathogenicity of PXO99 was stronger than that of C5. There were no remarkable differences in leaf microstructures among four O. officinalis populations, revealing the differences in resistance of four O. officinalis to BB are caused by the endogenous resistance genes. Furthermore, our results proved that there were no nine cloned BB resistance genes in four populations but possessed dominant Xa5, dominant Xa13, and recessive xa3/xa26 homologous alleles of xa5, xa13, and Xa3/Xa26 resistance genes. These three homologous genes were isolated and cloned from four populations and named OoXa5, OoXa13, and Ooxa3/xa26. The expression profile revealed that the expression levels of OoXa13 and Ooxa3/xa26 were significantly down-regulated under PXO99 and C5 stress, especially in the Gengma_Lincang population, suggesting the O. officinalis might enhance BB resistance by down-regulating the expression level of OoXa13 and Ooxa3/xa26. Conclusion The BB resistance genes of O. officinalis had its own characteristics by expression pattern and BLAST analysis of OoXa5, OoXa13, and Ooxa3/xa26, which indicated that there might be new genes or molecular mechanism of BB resistance in O. officinalis. Our studies provided a solid foundation and reference for revealing the molecular mechanism of BB resistance in O. officinalis.

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