文献类型： 外文期刊

作者： Wang, Guanbo ¹ ; Zhao, Haonan ¹ ; Zou, Jian ¹ ; Liang, Wenxing ¹ ; Zhao, Zhijian ³ ; Li, Delong ¹ ;

作者机构： 1.Engn Res Ctr Precis Pest Management Fruits & Veget, Coll Plant Hlth & Med, Qingdao, Peoples R China

2.Qingdao Agr Univ, Shandong Engn Res Ctr Environm Friendly Agr Pest M, Shandong Prov Key Lab Appl Mycol, Qingdao 266109, Peoples R China

3.Yunnan Acad Agr Sci, Ind Crops Inst, Kunming 650205, Peoples R China

关键词： Cytoplasmic coat protein complex II (COPII); ER-phagy; Autophagy; Sclerotia formation; Botrytis cinerea

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 263 卷

页码：

收录情况： SCI

摘要： Cytoplasmic coat protein complex II (COPII) plays a multifunctional role in the transport of newly synthesized proteins, autophagosome formation, and endoplasmic reticulum (ER)-ER-phagy. However, the molecular mechanisms of the COPII subunit in ER-phagy in plant pathogens remain unknown. Here, we identified the subunit of COPII vesicles (BcSfb3) and explored the importance of BcSfb3 in Botrytis cinerea. BcSfb3 deletion affected vegetative growth, conidiation, conidial morphology, and plasma membrane integrity. We confirmed that the increase in infectious hyphal growth was delayed in the Delta BcSfb3 mutant, reducing its pathogenicity in the host plant. Furthermore, the Delta BcSfb3 mutant was sensitive to ER stress, which caused massive ER expansion and induced the formation of ER whorls that were taken up into the vacuole. Further examination demonstrated that BcSfb3 deletion caused ER stress initiated by unfolded protein response, and which led to the promotion of ER-phagy and autophagy that participate in sclerotia formation. In conclusion, these results demonstrate that BcSfb3 plays an important role in fungal development, pathogenesis, ER-phagy and autophagy in B. cinerea.