Multiomics analysis of a resistant European turnip ECD04 during clubroot infection reveals key hub genes underlying resistance mechanism
文献类型： 外文期刊
作者： Zhou, Xueqing 1 ; Zhong, Ting 1 ; Wu, Meixiu 1 ; Li, Qian 1 ; Yu, Wenlin 1 ; Gan, Longcai 1 ; Xiang, Xianyu 1 ; Zhang, Yunyun 1 ; Shi, Yaru 1 ; Zhou, Yuanwei 5 ; Chen, Peng 1 ; Zhang, Chunyu 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China
3.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China
4.Yunnan Acad Agr Sci, Ind Crops Res Inst, Kunming, Peoples R China
5.Yichang Acad Agr Sci, Rice & Oil Res Inst, Yichang, Peoples R China
关键词： European fodder turnip; Plasmodiophora brassicae; transcriptome; miRNA; degradome; APS4
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： The clubroot disease has become a worldwide threat for crucifer crop production, due to its soil-borne nature and difficulty to eradicate completely from contaminated field. In this study we used an elite resistant European fodder turnip ECD04 and investigated its resistance mechanism using transcriptome, sRNA-seq, degradome and gene editing. A total of 1751 DEGs were identified from three time points after infection, among which 7 hub genes including XTH23 for cell wall assembly and two CPK28 genes in PTI pathways. On microRNA, we identified 17 DEMs and predicted 15 miRNA-target pairs (DEM-DEG). We validated two pairs (miR395-APS4 and miR160-ARF) by degradome sequencing. We investigated the miR395-APS4 pair by CRISPR-Cas9 mediated gene editing, the result showed that knocking-out APS4 could lead to elevated clubroot resistance in B. napus. In summary, the data acquired on transcriptional response and microRNA as well as target genes provide future direction especially gene candidates for genetic improvement of clubroot resistance on Brassica species.
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