文献类型： 外文期刊

作者： Zhou, Guoyan ¹ ; Zhang, Feifei ¹ ; Long, Wenjie ¹ ; Chen, Dan ¹ ; Wu, Xiaoyang ¹ ; Cai, Qing ¹ ; Wu, Shaoyun ¹ ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China; Minist Agr, Yunnan Prov Key Lab Agr Biotechnol, Key Lab Southwestern Crop Gene Resources & Germpl, Beijing, Peoples R China

关键词： Lipoxygenase activity; Hulled wheat; Relative expression level; Viability indices

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2019 年 21 卷 5 期

页码：

收录情况： SCI

摘要： This study investigated the changes in the viability indices (such as germination force, germination percent, weight germination index, seedling vigor index) of Lincang hulled wheat seeds after storage at different temperatures for different periods and determined the variations in the lipoxygenase (LOX) activity and the relative expression level (REL) of LOX gene. Lincang hulled wheat seeds were sealed in aluminum foil bags (10 cm x15 cm) and then stored in the gene bank at low-temperature below 0 degrees C (-7.5 +/- 2.5 degrees C, LT1), at low-temperature above 0 degrees C (7.5 +/- 2.5 degrees C, LT2), at the indoor temperature (ambient temperature, AT) and at high temperature (40 +/- 1 degrees C, HT) for 63, 125, 189, 210, 252 and 365 days. The viability indices including the LOX activity and the REL of LOX genes were then measured through germination test, spectrophotometer and quantitative real time polymerase chain reaction (qRT-PCR), respectively. The ANVOA, relativity and stepwise regression in SPSS software were carried out to analyze the data. The results indicated that viability indices of Lincang hulled wheat seeds after storage at LT1 and LT2 for 365 days showed no significant drop compared with seeds after storage at LT1 and LT2 for 252 days. The viability indices of seeds after storage at AT for 365 days were decreased significantly. After storage at HT for 14 d, all viability indices were significantly dropped compared with that were stored for 7 days. The LOX activity of germinated Lincang hulled wheat seeds as well as the relative expression level of LOX genes in leaves had a significant influence on the storage period of seeds. A higher LOX activity and a higher relative expression level of TaLOX2-5DL gene could lead to a short time storage of seeds at LT1; a higher relative expression level of TaLOX3-4A led to a shorter time storage at LT2 and AT; and a higher LOX activity led to a shorter storage period of seeds at HT. (C) 2019 Friends Science Publishers