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Diversity in flower colorations of Ranunculus asiaticus L. revealed by anthocyanin biosynthesis pathway in view of gene composition, gene expression patterns, and color phenotype

文献类型： 外文期刊

作者： Liu, Yanfang 1 ; Zhang, Jianhua 1 ; Yang, Xiaohong 1 ; Wang, Jiangmin 1 ; Li, Yangang 1 ; Zhang, Peng 1 ; Mao, Jin 1 ; Hu 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Minist Agr, DUS Test Kunming Stn, Kunming 650205, Yunnan, Peoples R China

2.Minist Agr, Dev Ctr Sci & Technol, Beijing 100122, Peoples R China

关键词： Ranunculus asiaticus L; Anthocyanin biosynthesis; Gene composition; Gene expression; Flower colorations

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

年卷期： 2019 年 26 卷 14 期

页码：

收录情况： SCI

摘要： Anthocyanin biosynthesis is one of the best studied secondary metabolisms. However, related pathways were generally concluded based on anthocyanin components; most studies focused on the backbone forming of anthocyanidins (cyanidin, delphinidin, and pelargonidin) of model or commercial plants, while anthocyanin modification was less discussed, and non-model plants with abundant colorations were less researched either. Ranunculus asiaticus L. has great diversity in flower colorations, not only indicating its value in researching anthocyanin biosynthesis but also implying it is unique in this regard. Based on transcriptome sequencing and gene annotation of three varieties (10 samples) of Ranunculus asiaticus L., 176 unigenes from 151,136 unigenes were identified as involved in anthocyanin biosynthesis, among which, 74 unigenes were related to anthocyanin modification; 61 unigenes were responsible for glycosylation at C3 and C5 with 3-monosaccharides of glucose, 3-biosides of rutinose, sophorose, or sambubiose to form 3Gly-, 3Gly5Gly-, 3Gly3Gly-, 3Gly2Gly-, 3Gly2Xly-, 3Gly2Rly-glycosylated anthocyanins, etc.; 2 unigenes transferred -CH3; 11 unigenes of BAHD family catalyzd the aromatic or malonyl acylation at 6 / 6position of 3/5-O-glucoside. Based on gene composition, a putative pathway was established. The pathway was validated by flower colorations, and gene expression patterns where F3H, F3H, 3GT, 5GT, and FMT2 were highly expressed in varieties colored as lateritious and carmine, while variety with purple flowers had high expression of F35H and 3MAT. In view of anthocyanin biosynthesis pathway of Ranunculus asiaticus L., great diversity in its flower colorations was illustrated via the complete branches (F3H, F3H and F35H) as well as complete modifications (glycosylation, methylation, and acylation), and besides, via the higher percentage of C3 glycosylation than C5 glycosylation.

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