Integrated Transcriptomic and Metabolomic Analyses Reveal the Importance of the Terpenoid, Fatty Acid, and Flavonoid Pathways in Rice Cell Death and Defense
文献类型： 外文期刊
作者： Bai, Pengfei 1 ; Liu, Yanfang 1 ; Gomes-Dias, Laisa 3 ; Combs-Giroir, Rachel 3 ; Dai, Shaoxing 7 ; Choi, Naeyeoung 1 ; Lin, Yun 3 ; Bernier, Matthew 8 ; Hatzakis, Emmanuel 9 ; Wang, Guo-Liang 1 ; Blakeslee, Joshua J. 3 ;
作者机构： 1.Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA
2.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650200, Peoples R China
3.Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA
4.Ohio State Univ, Lab Anal Metabolites Plants LAMP, Columbus, OH 43210 USA
5.Fed Univ Tocantins, Food Sci & Technol Program, BR-77001 Palmas, Brazil
6.Ohio State Univ, Ctr Appl Plant Sci, Columbus, OH 43210 USA
7.Kunming Univ Sci & Technol, Inst Primate Translat Med, Kunming 650500, Peoples R China
8.Ohio State Univ, Campus Chem Instrumentat Ctr CC, Columbus, OH 43210 USA
9.Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA
关键词： rice blast; cell death; metabolomics; transcriptomics; terpenoid; gibberellins
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 5 期
页码：
收录情况： SCI
摘要： Lesion mimic mutants provide unique tools to investigate plant-pathogen interactions, often exhibiting hypersensitive responses in the absence of biotic or abiotic stresses. The overexpression of the S-domain receptor-like kinase gene, SPL11 cell-death suppressor 2 (SDS2), in rice leads to constitutive programmed cell death and enhanced resistance to fungal and bacterial pathogens. However, the mechanisms underlying this broad-spectrum resistance remain unclear. This study integrates transcriptomic and metabolomic analyses of the SDS2-ACT mutant to uncover gene expression and metabolic shifts associated with disease resistance. To identify SDS2-specific physiological changes related to pathogen resistance, leaf tissues from the SDS2-ACT mutant and the Kitkaake WT line were subjected to both transcriptomic and non-targeted metabolic profiling. Transcriptomic analyses identified 1497 differentially expressed genes (DEGs), including up-regulated genes involved in terpenoid and flavonoid biosynthesis, phytohormone signaling, and defense-related pathways (including pathogenesis-related [PR] genes). Metabolomic profiling revealed significant alterations in the accumulation of several compound classes, including putative: terpenoids, phenylpropanoids, phytohormones, fatty acids, and sugars. These changes are likely correlated with the observed cell death and resistance phenotypes in the SDS2-ACT mutant. This study provides an overall landscape of the transcriptomic and metabolomic alterations in a lesion mimic mutant, identifying candidate defense-related genes and metabolites for functional analysis in rice.
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