Molecular Characterizations of the er1 Alleles Conferring Resistance to Erysiphe pisi in Three Chinese Pea (Pisum sativum L.) Landraces
文献类型: 外文期刊
作者: Sun, Suli 1 ; Deng, Dong 1 ; Wu, Wenqi 1 ; He, Yuhua 2 ; Luo, Gaoling 3 ; Du, Chengzhang 4 ; Duan, Canxing 1 ; Zhu, Zhendong 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
2.Yunnan Acad Agr Sci, Kunming 650205, Yunnan, Peoples R China
3.Guangxi Acad Agr Sci, Rice Res Inst, Nanning 530007, Peoples R China
4.Chongqing Acad Agr Sci, Inst Specialty Crop, Chongqing 402160, Peoples R China
关键词: Erysiphe pisi; er1-13; er1-14; KASPar marker; pea
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:6.208; 五年影响因子:6.628 )
ISSN:
年卷期: 2022 年 23 卷 19 期
页码:
收录情况: SCI
摘要: Powdery mildew caused by Erysiphe pisi DC. is a major disease affecting pea worldwide. This study aimed to confirm the resistance genes contained in three powdery mildew-resistant Chinese pea landraces (Suoshadabaiwan, Dabaiwandou, and Guiwan 1) and to develop the functional markers of the novel resistance genes. The resistance genes were identified by genetic mapping and PsMLO1 gene sequence identification. To confirm the inheritance of powdery mildew resistance in the three Landraces, the susceptible cultivars Bawan 6, Longwan 1, and Chengwan 8 were crossed with Suoshadabaiwan, Dabaiwandou, and Guiwan 1 to produce F-1, F-2, and F-2:3 populations, respectively. All F-1 plants were susceptible to E. pisi, and phenotypic segregation patterns in all the F-2 and F-2:3 populations fit the 3:1 (susceptible: resistant) and 1:2:1 (susceptible homozygotes: heterozygotes: resistant homozygotes) ratios, respectively, indicating powdery mildew resistance in the three Landraces were controlled by a single recessive gene, respectively. The analysis of er1-linked markers and genetic mapping in the F-2 populations suggested that the recessive resistance genes in three landraces could be er1 alleles. The cDNA sequences of 10 homologous PsMLO1 cDNA clones from the contrasting parents were obtained. A known er1 allele, er1-4, was identified in Suoshadabaiwan. Two novel er1 alleles were identified in Dabaiwandou and Guiwan 1, which were designated as er1-13 and er1-14, respectively. Both novel alleles were characterized with a 1-bp deletion (T) in positions 32 (exon 1) and 277 (exon 3), respectively, which caused a frame-shift mutation to result in premature termination of translation of PsMLO1 protein. The co-dominant functional markers specific for er1-13 and er1-14, KASPar-er1-13, and KASPar-er1-14 were developed and effectively validated in populations and pea germplasms. Here, two novel er1 alleles were characterized and their functional markers were validated. These results provide powerful tools for marker-assisted selection in pea breeding.
- 相关文献
作者其他论文 更多>>
-
Production status and research advancement on root rot disease of faba bean (Vicia faba L.) in China
作者:Yu, Haitian;Yang, Feng;Hu, Chaoqin;Yang, Xin;Zheng, Aiqing;Wang, Yubao;He, Yuhua;Lv, Meiyuan;Yu, Haitian;Tang, Yongsheng
关键词:Vicia faba; production; root rot; identification; management
-
Yield Adaptability and Stability in Field Pea Genotypes Using AMMI, GGE, and GYT Biplot Analyses
作者:Yang, Xin;Soliman, Alaa A.;Hu, Chaoqin;Yang, Feng;Lv, Meiyuan;Yu, Haitian;Wang, Yubao;Zheng, Aiqing;Dai, Zhengming;Li, Qiong;Wang, Liping;He, Yuhua;Soliman, Alaa A.;Tang, Yongsheng;Yang, Jiangu;Zhang, Yurong;Niu, Wenwu
关键词:pea; G x E interaction; stability; AMMI; GGE biplot; GYT biplot; seed yield; abiotic stress
-
The Potential Genetic Effect for Yield and Foliar Disease Resistance in Faba Bean (Vicia faba L.) Assessed via Morphological and SCoT Markers
作者:Soliman, Alaa A.;Mousa, Manar I.;Soliman, Alaa A.;Yu, Haitian;He, Yuhua;Mosalam, Abeer M.;Ghareeb, Zeinab E.;Ibrahim, Shafik D.;Rehan, Medhat;Rehan, Medhat
关键词:genetic variability; heterosis; heritability; genetic advance; Vicia faba; molecular markers; SCoT; foliar diseases
-
Screening for Pea Germplasms Resistant to Fusarium Wilt Race 5
作者:Deng, Dong;Sun, Suli;Wu, Wenqi;Zong, Xuxiao;Duan, Canxing;Zhu, Zhendong;Yang, Xiaoming;Zhang, Xiaoyan;He, Yuhua
关键词:Pisum sativum; Fusarium wilt; Fusarium oxysporum f. sp. pisi; race; resistance identification
-
Performance of Different Varieties of Spring Field Pea (Pisum sativum L.) under Irrigated and Rainfed Environments in North China
作者:Min, Gengmei;Zhang, Lijuan;Gou, Zhiwen;Yang, Jingyi;He, Yuhua;Zong, Xuxiao;Lian, Rongfang;Liu, Zhenxing;Xiang, Chao;Li, Ling;Xing, Baolong
关键词:multi-environment yield trials; pea; genotypes; grain yield; adaptability
-
Pathogenicity, anastomosis groups, host range, and genetic diversity of Rhizoctonia species isolated from soybean, pea, and other crops in Alberta and Manitoba, Canada
作者:Yu, Haitian;He, Yuhua;Zhou, Qixing;Hwang, Sheau-Fang;Chang, Kan-Fa;Hwang, Sheau-Fang;Ho, Andrew J.;Strelkov, Stephen E.;Conner, Robert L.;Harding, Michael W.
关键词:Rhizoctonia; soybean; pea; pathogenicity; anastomosis group; ITS; ISSR
-
Economic Assessment of Food Legumes Breeding in China: Evidence Using a Provincial Level Dataset
作者:Ma, Jiliang;Zhang, Huijie;Khan, Nawab;Tian, Jing;Wang, Lixia;Wu, Jing;Cheng, Xuzhen;Ren, Guixing;Chen, Xin;Liu, Yujiao;He, Yuhua;Liu, Changyan;Xia, Xianfei;Guo, Yanping;Zhang, Xiaoyan;Zhou, Bin;Liu, Zhenxing;Lian, Rongfang
关键词:food legumes; breeding; research and development; China; global Moran's I index