您好，欢迎访问云南省农业科学院 机构知识库！

Regulation Mode of RaMYB1 of Ranunculus asiaticus L. in Anthocyanin Biosynthesis Responding to Temperature Decrease

文献类型： 外文期刊

作者： Liu, Yanfang 1 ; Yang, Xiaohong 1 ; Huang, Qingmei 1 ; Qiao, Juxiang 1 ; Yang, Xuhong 2 ;

作者机构： 1.Yunnan Acad Agr Sci, Distinctness Uniform Stabil Test Kunming Stn, Minist Agr, Qual Stand & Testing Technol Res Inst, Kunming 650205, Yunnan, Peoples R China

2.Minist Agr, Dev Ctr Sci & Technol, Beijing 100122, Peoples R China

关键词： Flower Color; Ranunculus asiaticus L; Anthocyanin Biosynthesis; Transcription Regulation; MYB; Cold Stress; Target Genes

期刊名称：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY （ 影响因子：0.687； 五年影响因子：0.558 ）

ISSN： 1556-6560

年卷期： 2021 年 15 卷 6 期

页码：

收录情况： SCI

摘要： Color is of great importance for the testing and breeding of plant varieties, and is known to be varied along with temperature changes. However, at the transcription level, the exact binding sites and corresponding regulation properties in the pathway of anthocyaninn biosynthesis in vivo are rarely reported. In this study, variety Doppio Pandora-purple of Ranunculus asiaticus L. whose flower color is sensitive to the decrease in temperature was used. In the results, 27 genes involved in anthocyanin biosynthesis were differentially expressed under cold stress, and 7 MYB genes were up-regulated; the most obviously up-regulated MYB (RaMYB1) gene belongs to R2R3-MYB type. Promoter sequences of the 27 genes were concluded from transcriptome sequences and genome walking, for the purpose of sequence alignment with ChIP-seq/DAP-seq sequences. 7 genes were determined as target genes of RaMYB1 in cold-induced transcription regulation, including 2 PAL (phenylalanine ammonia lyase), 2 CHS (chalcone synthase), 1 F3H (flavanone 3-hydroxylase), 1 DFR (dihydroflavonol 4-reductase), 1 F3H (flavanoid 3-hydroxylase). ChIP-PCR indicated that, the binding of RaMYB1 to DFR can be induced by temperature decrease, while the bindings to other 6 genes were constitutive and can be strengthened at 5 degrees C decrease. Regulation properties of RaMYB1 to the 7 genes were determined as enhancement due to their up-regulation as well as the enhancer in C terminal of RaMYB1. Regulation mode of RaMYB1 was mapped accordingly and validated via HPLC-based anthocyanin content, gene expression level and coloration. The regulation mode can illustrate the binding sites and enhancement property of RaMYB1 in the regulation of anthocyanin biosynthesis under cold stress. It can be applied in color-oriented temperature monitoring and in molecular breeding. The way to establishing the regulation mode may be interest for other non-model plant genera with little molecular background.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台