文献类型： 外文期刊

作者： Cheng, Z. ¹ ; Tang, K. ¹ ; Yan, H. ¹ ; Fu, J. ¹ ; Ying, F. ¹ ; Huang, X. ¹ ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Genet Germplasm Inst, Kunming 650223, Peoples R China

2.Yunnan Univ, Coll Life Sci, Dept Biochem & Mol Biol, Kunming 650221, Peoples R China

关键词： cDNA: complementary DNA;peroxide: 14915-07-2;Gene Name;Oryza meyeriana ME281 gene [Gramineae]: expression;molecular mechanism;Genbank sequence data;signal transduction pathway;disease resistance;cell wall lignification;secondary metabolism system

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

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收录情况： SCI

摘要： P>Oryza meyeriana Baill was found immune to Xanthomonas oryzae pv. oryzae (Xoo). However, the genes responsible for the strong resistance are unknown. In this study, suppression subtractive hybridization (SSH) was utilized to identify genes differentially expressed in O. meyeriana after Xoo infection. Oryza meyeriana plants inoculated with high pathogenicity races were used as 'tester' and non-inoculated plants as 'driver'. Analysis of the SSH library revealed genes responsible for cell wall lignifications, which may function to block pathogen invasion. Also identified are genes involved in secondary metabolism systems to eliminate damage caused by chemicals such as peroxides in cells. One of the response genes, ME281 encoding a protein with leucine-rich repeats which has high similarity with those of other reported disease resistance genes, was further analysed by RT-PCR and Northern blotting, which showed its expression peaked at 48-72 h after Xoo infection, suggesting that it may function at the later stage of the plant's responses. This study would help demonstrate the molecular mechanism of O. meyeriana's strong resistance to Xoo and find new disease resistance candidate genes.