Quantification of lutein and zeaxanthin in marigold (Tagetes erecta L.) and poultry feed by ultra-performance liquid chromatography and high performance liquid chromatography
文献类型： 外文期刊
作者： Liu, Hongcheng 1 ; Zhang, Ying 2 ; Li, Qiwan 1 ; Zou, Yanhong 1 ; Shao, Jinliang 1 ; Lan, Shanshan 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Qual Standard & Testing Technol, Supervis & Testing Ctr Farm Prod Qual, Minist Agr, Kunming, Yunnan Province, Peoples R China
2.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310003, Zhejiang, Peoples R China
关键词： HPLC;hydrolysis;lutein;UPLC;zeaxanthin
期刊名称：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES （ 影响因子：1.312； 五年影响因子：1.145 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A simple, reliable, and rapid hydrolysis method for quantitative determination of lutein and zeaxanthin in marigold and poultry feed was developed and analyzed by UPLC. Faster analysis time and better resolution were obtained by analysis columns with 1.7-m particle materials in UPLC. The mean recovery for marigold was 85.4%-100% and for poultry feed was 83.4%-106.3%, relative standard deviation (RSD) was 8.75% or less. The concentrations of lutein and zeaxanthin in 20 marigold samples were assessed by the UPLC method and the HPLC method. The regression coefficients between concentrations of lutein obtained under the two methods were close to 1, and the regression coefficients of zeaxanthin were 0.96. Limit of detection (LOD) and limit of quantification (LOQ) of lutein in the two methods were the same values of 0.02mg/100g and 0.1mg/100g, whereas LOD and LOQ for zeaxanthin in UPLC were twofold lower than values of 0.04mg/100g and 0.2mg/100g in HPLC.
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