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DETECTION OF 'CANDIDATUS LIBERIBACTER ASIATICUS' IN CACOPSYLLA (PSYLLA) CITRISUGA (HEMIPTERA: PSYLLIDAE)

文献类型： 外文期刊

作者： Cen, Yijing 1 ; Zhang, Lina 1 ; Xia, Yulu 2 ; Guo, Jun 3 ; Deng, Xiaoling 1 ; Zhou, Wenjing 1 ; Sequeira, Ronald 2 ; Gao, 1 ;

作者机构： 1.S China Agr Univ, Citrus Huanglongbing Res Lab, Lab Insect Ecol, Guangzhou 510642, Guangdong, Peoples R China

2.N Carolina State Univ, Ctr Integrated Pest Management, Raleigh, NC 27606 USA

3.Yunnan Acad Agr Sci, Ruili Expt Stn, Inst Trop & Subtrop Cash Crops, Yunnan Ruili 678600, Peoples R China

4.Yunnan Acad Agr Sci, Ruili Expt Stn, Inst Trop & Subtrop Cash Crops, Yunnan Ruili 678600, Peoples R

关键词： pomelo psyllid;Huanglongbing;PCR;‘Candidatus Liberibacter asiaticus’;detection

期刊名称：FLORIDA ENTOMOLOGIST （ 影响因子：1.425； 五年影响因子：1.595 ）

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收录情况： SCI

摘要： Nymphs of the pomelo psyllid, Cacopsylla (Psylla) citrisuga Yang & Li, were collected from huanglongbing (HLB) symptomatic lemon trees, Citrus limon (L.) in Yunnan Province, China. DNA samples extracted from groups of 2-10 pomelo psyllid nymphs of all stages and from leaves of lemon plants were analyzed with nested-PCR in order to detect 'Candidatus Liberibacter asiaticus' (Las). The results showed that 24 out of the 49 lemon trees were Las-positive. Also psyllid nymphs collected from 10 out of the 24 Las-positive plants were Las-positive. DNA extracted from individual late stage nymphs (3rd to 5th instars) collected from Las-infected trees were then subjected to nested-PCR trials. Twelve out of the 29 nymphs proved to be Las positive. All nymphs collected from Las-negative lemon trees were also Las-negative. Most psyllid samples which showed positive in nested-PCR were also positive in conventional-PCR detection. The amplified fragment of the 16S rRNA gene of 'Ca. Liberibacter spp.' from positive psyllid samples was 99% similar to those of Las strain psy62 in GenBank. These results demonstrate that Cacopsylla (Psylla) citrisuga is another Las carrier insect. Transmission studies are underway to determine whether Las-positive psyllids can transmit Las to healthy citrus.

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