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A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa

文献类型： 外文期刊

作者： Sun, Jian 1 ; Zhao, Yongchang 3 ; Chai, Hongmei 3 ; Wang, Hexiang 1 ; Ng, Tzi Bun 4 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China

3.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming, Peoples R China

4.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

关键词： Alkaline protease;Amanita farinosa;Antiproliferative;Fruiting bodies;Mushroom;Purification

期刊名称：ACTA BIOCHIMICA POLONICA （ 影响因子：2.149； 五年影响因子：2.175 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A novel protease with a molecular mass of 15 kDa was purified from fresh fruiting bodies of the wild mushroom Amanita farinosa. The purification protocol entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The protease was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and SP-Sepharose. It demonstrated a single 15-kDa band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/ PAGE) and a 15-kDa peak in gel filtration. The optimal pH and optimal temperature of the protease were pH 8.0 and 65 °C, respectively. Proliferation of human hepatoma HepG2 cells was inhibited by the protease with an IC _50 of 25 μM. The protease did not have antifungal or ribonuclease activity.

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