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Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners

文献类型： 外文期刊

作者： Zhang, Mengru 1 ; Gong, Ming 1 ; Yang, Yumei 1 ; Li, Xujuan 2 ; Wang, Haibo 3 ; Zou, Zhurong 1 ;

作者机构： 1.Yunnan Normal Univ, Key Lab Biomass Energy & Environm Biotechnol Yunn, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ,Sch Life Sci, Kunming 650500, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Yunnan Key Lab Sugarcane Genet Improvement, Sugarcane Res Inst, Kaiyuan 661600, Yunnan, Peoples R China

3.Qujing Normal Univ, Coll Biol Resources & Environm Sci, Qujing 655011, Yunnan, Peoples R China

关键词： Ascorbate peroxidase 1;Fusion partner;Heat-susceptibility;Hyper-acidity;Jatropha curcas;Thermostability

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： Cytosolic ascorbate peroxidase 1 (APX1) plays a crucial role in regulating the level of plant cellular reactive oxygen species and its thermolability is proposed to cause plant heat-susceptibility. Herein, several hyper-acidic fusion partners, such as the C-terminal peptide tails, were evaluated for their effects on the thermal stability and activity of APX1 from Jatropha curcas and Arabidopsis. The hyper-acidic fusion partners efficiently improved the thermostability and prevented thermal inactivation of APX1 in both plant species with an elevated heat tolerance of at least 2 A degrees C. These hyper-acidified thermostable APX1 fusion variants are of considerable biotechnological potential and can provide a new route to enhance the heat tolerance of plant species especially of inherent thermo-sensitivity.

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