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Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in non-heading Chinese cabbage

文献类型： 外文期刊

作者： Li, Xia 1 ; Wang, Aihua 1 ; Zu, Feng 3 ; Hu, Zhenhua 1 ; Lin, Jiazao 1 ; Zhou, Guolin 1 ; Tu, Jinxing 2 ;

作者机构： 1.Wuhan Acad Agr Sci & Technol, Wuhan Vegetable Res Inst, Wuhan 430345, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

3.Yunnan Acad Agr Sci, Ind Crop Res Inst, Kunming 650223, Peoples R China

关键词： Non-heading Chinese cabbage;Male sterility;Brassica 60 K SNP array;Molecular marker

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

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收录情况： SCI

摘要： WS24-3 is a newly bred recessive genic male sterility line of the non-heading Chinese cabbage (Brassica rapa ssp. chinensis). Here, an F-2 population was produced from the cross between WS24-3 and a male-fertile breeding line (WS135). The Illumina Brassica 60 K single nucleotide polymorphism (SNP) array was used for SNPs detecting between sterile and fertile bulks from the F2 population, and 62 SNPs were identified. BLAST analysis of the 62 SNPs revealed that the A2 chromosome of Brassica rapa genome contained 22 SNPs, whereas the other chromosomes did not contain more than 6 SNPs each. These data indicated that the potential target gene locus, named Bra2Ms, might be located on A2. Based on 10 of the 22 SNPs, allele-specific-polymerase chain reaction (AS-PCR) primers and single sequence repeat (SSR) primers were designed, 5 AS-PCR primers and 9 SSR primers showed difference between the bulks in electrophoretic determination. Analysis of these markers in F2 population revealed that Bra2Ms was genetically delimited to a region of 1.2 cM. We also detected two co-segregated markers SSRa2-951 and SSRa2-960 in this region. The markers identified in our study might facilitate the transfer of recessive genic male sterility alleles to other favorable genetic backgrounds. Furthermore, these markers will support a map-based clone of Bra2Ms.

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