Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in non-heading Chinese cabbage
文献类型： 外文期刊
作者： Li, Xia 1 ; Wang, Aihua 1 ; Zu, Feng 3 ; Hu, Zhenhua 1 ; Lin, Jiazao 1 ; Zhou, Guolin 1 ; Tu, Jinxing 2 ;
作者机构： 1.Wuhan Acad Agr Sci & Technol, Wuhan Vegetable Res Inst, Wuhan 430345, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
3.Yunnan Acad Agr Sci, Ind Crop Res Inst, Kunming 650223, Peoples R China
关键词： Non-heading Chinese cabbage;Male sterility;Brassica 60 K SNP array;Molecular marker
期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： WS24-3 is a newly bred recessive genic male sterility line of the non-heading Chinese cabbage (Brassica rapa ssp. chinensis). Here, an F-2 population was produced from the cross between WS24-3 and a male-fertile breeding line (WS135). The Illumina Brassica 60 K single nucleotide polymorphism (SNP) array was used for SNPs detecting between sterile and fertile bulks from the F2 population, and 62 SNPs were identified. BLAST analysis of the 62 SNPs revealed that the A2 chromosome of Brassica rapa genome contained 22 SNPs, whereas the other chromosomes did not contain more than 6 SNPs each. These data indicated that the potential target gene locus, named Bra2Ms, might be located on A2. Based on 10 of the 22 SNPs, allele-specific-polymerase chain reaction (AS-PCR) primers and single sequence repeat (SSR) primers were designed, 5 AS-PCR primers and 9 SSR primers showed difference between the bulks in electrophoretic determination. Analysis of these markers in F2 population revealed that Bra2Ms was genetically delimited to a region of 1.2 cM. We also detected two co-segregated markers SSRa2-951 and SSRa2-960 in this region. The markers identified in our study might facilitate the transfer of recessive genic male sterility alleles to other favorable genetic backgrounds. Furthermore, these markers will support a map-based clone of Bra2Ms.
- 相关文献
作者其他论文 更多>>
-
Health-promoting compounds in Amomum villosum Lour and Amomum tsao-ko : Fruit essential oil exhibiting great potential for human health
作者：Imran, Sehrish;Bibi, Yamin;Yang, Li-E;Yang, Jiazhen;Yang, Xiaomeng;Pu, Xiaoying;Li, Xia;Zeng, Yawen;Qayyum, Abdul;He, Wei
关键词：Zingiberaceae; Amomum; Camphor; Borneol; Nutraceutical; Neuroprotective
-
Evolution and association analysis of SSIIIa in rice landraces of Yunnan Province
作者：Li, Xia;Yang, Xiaomeng;Yang, Li'e;Yang, Jiazhen;Yang, Tao;Pu, Xiaoying;Zeng, Yawen;Ahmed, Hafiz Ghulam Muhu-Din;Yao, Chunlian;Wang, Luxiang
关键词：Association analysis; Resistant starch; SSIIIa; Rice landraces; Natural variation
-
Fine mapping and cloning of the sterility gene Bra2Ms in nonheading Chinese cabbage (Brassica rapa ssp. chinensis)
作者：Song, Liping;Tang, Liguang;Yu, Chuying;Wang, Bincai;Gao, Changbin;Xie, Yanfeng;Zhang, Xueli;Wang, Junliang;Lin, Chufa;Wang, Aihua;Li, Xia
关键词：non-heading Chinese cabbage; male sterility; Bra2Ms; fine mapping; PHD-finger protein
-
Physiological Mechanisms by Which the Functional Ingredients in Beer Impact Human Health
作者：Zeng, Yawen;Li, Xia;Yang, Li'e;Pu, Xiaoying;Yang, Xiaomeng;Yang, Tao;Ahmed, Hafiz Ghulam Muhu-Din;Yang, Jiazhen
关键词：functional ingredients; actional mechanism; barley grains and grass; beer; malt; hops; functional foods; chronic diseases; human
-
Development of Genome-Wide Intron Length Polymorphism (ILP) Markers in Tea Plant (Camellia sinensis) and Related Applications for Genetics Research
作者：Shen, Yuan;Huang, Xiaoxia;Yin, Shihua;Wang, Lifei;Geng, Fang;Cheng, Xiaomao;He, Xiaoying;Zu, Feng
关键词：Camellia tetracocca; intron length polymorphism (ILP); transferability; genetic diversity; population structure
-
Geographic differences and variation of functional components of brown rice in 690 mini-core collections from global germplasms
作者：Yang, Xiaomeng;Mandal, Md Siddikun Nabi;Du, Juan;Pu, Xiaoying;Li, Xia;Yang, Jiazhen;Zeng, Yawen;Mandal, Md Siddikun Nabi;Hossain, Akbar;Diao, Henan;Li, Jianbin;Diao, Henan;Li, Zichao;Ali, Muhammad Kazim
关键词：Brown rice; Mini-core collection; Functional components; Genotypic differences; Regional characteristics
-
Integrating biochemical and anatomical characterizations with transcriptome analysis to dissect superior stem strength of ZS11 (Brassica napus)
作者：Tian, Zhengshu;Wang, Xinfa;Dun, Xiaoling;Tian, Ze;Zhang, Xiaoxue;Ren, Lijun;Wang, Hanzhong;Tian, Zhengshu;Tu, Jinxing;Tian, Zhengshu;Li, Jinfeng;Wang, Xinfa;Wang, Hanzhong
关键词：Brassica napus; stem strength; rind penetrometer resistance; cell wall components; S-adenosylmethionine
