文献类型： 外文期刊
作者： Pan, Qiuling 1 ; Wang, Ling 1 ; Dang, Xiaoqun 1 ; Ma, Zhengang 1 ; Zhang, Xiaoyan 1 ; Chen, Shiliang 2 ; Zhou, Zeyang; 1 ;
作者机构： 1.Chongqing Normal Univ, Coll Life Sci, 37 Middle Rd, Chongqing 401331, Peoples R China
2.Yunnan Acad Agr Sci, Sericultural & Apicultural Inst, Mengzi 661101, Yunnan, Peoples R China
3.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400716, Peoples R China
关键词： ADP;ATP carrier protein;domestic silkworm;RNA interference
期刊名称：JOURNAL OF EUKARYOTIC MICROBIOLOGY （ 影响因子：3.346； 五年影响因子：2.809 ）
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收录情况： SCI
摘要： The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain (DE3) to express double-strand RNAs targeting the gene encoding ADP/ATP protein in N.bombycis. The results showed that dsRNAs deferentially suppressed the gene expression during N.bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the dsRNA expressed by recombinant E.coli to control the pebrine disease of the domestic silkworm.
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