Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors
文献类型： 外文期刊
作者： Li, Jinxin 1 ; Li, Hongfa 1 ; Liu, Dahui 3 ; Liu, Shujie 2 ; Li, Jianli 2 ; Wang, Juan 1 ;
作者机构： 1.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China
2.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China
3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650231, Peoples R China
关键词： RT-PCR;SDS-PAGE;Antioxidant enzymes;Adventitious roots;HPLC-ESI-MSn
期刊名称：RESEARCH ON CHEMICAL INTERMEDIATES （ 影响因子：2.914； 五年影响因子：2.32 ）
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收录情况： SCI
摘要： In this study, Candida tropicalis, Aspergillus niger, and a mixture of their cultures were previously used as fungal elicitors in Panax quinquefolium adventitious root culture to enhance ginsenoside production. The result showed that ginsenoside content (11.47 +/- 0.54 mg g(-1)) was significantly enhanced to 3.25-fold of the control group after treatment with 400 mg L-1 C. tropicalis. The polysaccharide content (213.01 +/- 18.63 mg g(-1)) reached the highest when A. niger was at the concentration of 200 mg L-1. HPLC-ESI-MSn analysis was performed, showing that ginsenosides Rh-1 and Ro were identified after treatment with fungal elicitors. Furthermore, we found that C. tropicalis, A. niger, and mix elicitor significantly up-regulated the expression of the squalene synthase, squalene epoxidase, dammarenediol synthase, beta-amyrin synthase, protopanaxadiol synthase, protopanaxatriol synthase, and UDP-glycosyltransferase. SDS-polyacrylamide gel electrophoresis was used to analysis the changes of protein. A new band was discovered at the size of 75 kDa. In addition, it found that fungal elicitors significantly increased the activities of catalases and peroxidase.
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