云南省农业科学院机构知识库

Determination of Iridoids in Gentiana rigescens by Infrared Spectroscopy and Multivariate Analysis

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文献类型：外文期刊

作者： Qi, Lu-Ming ¹ ; Zhang, Ji ² ; Zuo, Zhi-Tian ² ; Zhao, Yan-Li ² ; Wang, Yuan-Zhong ² ; Hang, Jin ² ;

作者机构： 1.Yunnan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Kunming, Peoples R China

2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming, Peoples R China

3.Yunnan Tech Ctr Qual Chinese Mat Med, Kunming, Peoples R China

关键词： Determination;Gentiana rigescens;infrared spectroscopy;iridoids;partial least squares

期刊名称：ANALYTICAL LETTERS （影响因子：2.329；五年影响因子：1.738 ）

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年卷期：

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收录情况： SCI

摘要： Gentiana rigescens is a famous herbal medicine in China for treatment of convulsion, rheumatism, and jaundice. Here, the infrared determination of gentiopicroside, swertiamarin, sweroside, and loganic acid in G. rigescens from different areas and varieties was presented for the first time. Reference information for the iridoids were obtained by high-performance liquid chromatography. Partial least squares was used to characterize the relationship between spectra matrix and concentration vector for the determination of the analytes. For determination of gentiopicroside, the appropriate performance of partial least squares model was acquired with coefficient of determination of calibration and coefficient of determination of prediction values of 0.965 and 0.868. The root mean square error of estimation (RMSEE), root mean square error of cross validation (RMSECV), root mean square error of prediction (RMSEP), and residual predictive deviation (RPD) values were 2.612, 5.292, 5.239mg g(-1), and 2.701, respectively, based on the first derivative and multiplicative scatter correction. For determination of the total iridoids, the best results were obtained using the coefficient of determination of calibration and coefficient of determination of prediction of 0.943 and 0.834, RMSEE, RMSECV, RMSEP and RPD of 3.896, 7.536, 6.543mg g(-1) and 2.438, respectively, based on the first derivative. Both models were reliable and robust. The results demonstrated that infrared spectroscopy provided a rapid, low-cost tool to monitor the quality of G. rigescens by the determination of the iridoids.

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意见箱

Determination of Iridoids in Gentiana rigescens by Infrared Spectroscopy and Multivariate Analysis

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意 见 箱

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意见箱