文献类型： 外文期刊

作者： Wang, J. W. ¹ ; Tian, H. ¹ ; Yu, X. ¹ ; Zheng, L. P. ³ ;

作者机构： 1.Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Peoples R China

2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650205, Peoples R China

3.Soochow Univ, Sch Architecture, Dept Hort, Suzhou 215123, Peoples R China

关键词： hydrogen peroxide;NADPH oxidase;nitrate reductase;nitric oxide;reactive oxygen species

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

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收录情况： SCI

摘要： Artemisinin, a natural sesquiterpenoid isolated from Artemisia annua L., is regarded as the most efficient drug against malaria in the world. Artemsinin production in NaCl-treated A. annua seedlings and its relationships with the glucose-6-phosphate dehydrogenase (G6PDH) activity and generation of H2O2 and nitric oxide (NO) were investigated. Results revealed that artemisinin content in the seedlings was increased by 79.3 % over the control after 1-month treatment with 68 mM NaCl. The G6PDH activity was enhanced in the presence of NaCl together with stimulated generation of H2O2 and NO. Application of 1.0 mM glucosamine (GlcN), an inhibitor of G6PDH, blocked the increase of NADPH oxidase and nitrate reductase (NR) activities, as well as H2O2 and NO production in A. annua seedlings under the salt stress. The induced H2O2 was found to be involved in the upgrading gene expression of two key enzymes in the later stage of artemisinin biosynthetic pathway: amorphadiene synthase (ADS) and amorpha-4,11-diene monooxygenase (CYP71AV1). The released NO being attributed mainly to the increase of NR activity, negatively interacted with H2O2 production and enhanced gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR). Inhibition of NO generation partly blocked NaCl-induced artemisinin accumulation, and NO donor strongly rescued the decreased content of artemisinin caused by GlcN. These results suggest that G6PDH could play a critical role in NaCl-induced responses and artemisinin biosynthesis in A. annua.