Quantitative determination and evaluation of Paris polyphylla var. yunnanensis with different harvesting times using UPLC-UV-MS and FT-IR spectroscopy in combination with partial least squares discriminant analysis
文献类型： 外文期刊
作者： Yang, Yuan-Gui 1 ; Zhang, Ji 2 ; Zhao, Yan-Li 2 ; Zhang, Jin-Yu 2 ; Wang, Yuan-Zhong 2 ;
作者机构： 1.Yunnan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Kunming, Peoples R China
2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Peoples R China
3.Yunnan Tech Ctr Qual Chinese Mat Med, Kunming, Peoples R China
关键词： FT-IR;harvesting time;Paris polyphylla var. yunnanensis;partial least squares discriminant analysis;UPLC-UV-MS
期刊名称：BIOMEDICAL CHROMATOGRAPHY （ 影响因子：1.902； 五年影响因子：1.809 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： A rapid method was developed and validated by ultra-performance liquid chromatography-triple quadrupole mass spectroscopy with ultraviolet detection (UPLC-UV-MS) for simultaneous determination of paris saponin I, paris saponin II, paris saponin VI and paris saponin VII. Partial least squares discriminant analysis (PLS-DA) based on UPLC and Fourier transform infrared (FT-IR) spectroscopy was employed to evaluate Paris polyphylla var. yunnanensis (PPY) at different harvesting times. Quantitative determination implied that the various contents of bioactive compounds with different harvesting times may lead to different pharmacological effects; the average content of total saponins for PPY harvested at 8years was higher than that from other samples. The PLS-DA of FT-IR spectra had a better performance than that of UPLC for discrimination of PPY from different harvesting times.
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