您好，欢迎访问云南省农业科学院 机构知识库！

Cloning and Expression of Omission of Second Division-like Genes from Carnation

文献类型： 外文期刊

作者： Zhou, Xuhong 1 ; Mo, Xijun 1 ; Jiang, Yalian 1 ; Zhang, Hao 1 ; Yu, Rongpei 1 ; Wang, Lihua 1 ; Wang, Jihua 1 ; Qu, Supin 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China; Natl Engn Res Ctr Ornamental Hort, Kunming 650205, Yunnan, Peoples R China

关键词： Dianthus caryophyllus;OSD1;meiosis;2n pollen;mechanism;polyploidization

期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.144； 五年影响因子：1.617 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The omission of second division gene (OSD1) gene plays a fundamental role in meiosis and is associated with 2n gamete formation in Arabidopsis thaliana. The objective of this work was to unravel the mechanisms leading to 2n pollen production, and isolate and analyze the expression patterns of OSD-like (OSDL) genes in carnation (Dianthus caryophyllus). We found an absence of the second meiotic division caused the formation of 2n pollen. Three homoeologous genes were cloned and labeled as OSDLa, OSDLb, and OSDLc in a diploid carnation. The cDNAs were 1180 bp for OSDLa, 1288 bp for OSDLb, and 971 bp for OSDLc. A strong similarity was found between the amino sequences of OSDLb and OSDLc. An evident feature of OSDLs proteins is the presence of D-box and MR-tail domains; however, the GxEN/KEN-box domain, which is distinct among the other plant proteins was absent. Quantitative real time polymerase chain reaction (qRT-PCR) analysis showed that OSDL genes maintain continuous expression in buds and other tissues. OSDLa has the highest expression in buds of 1.1-1.2 cm long (stage 2), and OSDLb has a high level of expression in buds of 0.9-1.0 cm long (stage 1) and stage 2 buds and ovary tissues in three carnation cultivars. The expression level of OSDLc was highest in ovaries. These expression patterns strongly suggest that OSDLs in carnation involve male meiosis and ovary development. These findings can have potential applications in fundamental polyploidization research and plant breeding programs in carnation.

  • 相关文献

[1]The biological characters and polyploidy of progenies in hybridization in 4x-2x crosses in Dianthus caryophyllus. Zhou, Xuhong,Su, Yan,Yang, Xiumei,Zhang, Yiping,Li, Shengchong,Gui, Min,Wang, Jihua.

[2]Karyological diversity of wild Rosa in Yunnan, southwestern China. Jian, Hong Ying,Zhang, Ting,Wang, Qi Gang,Li, Shu Bin,Zhang, Hao,Tang, Kai Xue.

[3]Cytological, molecular mechanisms and temperature stress regulating production of diploid male gametes in Dianthus caryophyllus L.. Zhou, Xuhong,Luo, Ying,Tang, Wenru,Zhou, Xuhong,Luo, Ying,Tang, Wenru,Zhou, Xuhong,Mo, Xijun,Gui, Min,Wu, Xuewei,Jiang, Yalian,Ma, Lulin,Shi, Ziming,Zhou, Xuhong,Mo, Xijun,Gui, Min,Wu, Xuewei,Jiang, Yalian,Ma, Lulin,Shi, Ziming.

[4]In vitro Antimicrobial Activities and Mechanism of 1-Octen-3-ol against Food-related Bacteria and Pathogenic Fungi. Li, Qiang,Chen, Zuqin,Xiong, Chuan,Li, Qiang,Huang, Wenli,Li, Shuhong,Chen, Cheng. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台