文献类型： 外文期刊

作者： Gu, Kaiyuan ¹ ; Yang, Li-E. ² ; Ren, Ke ¹ ; Luo, Xianxue ³ ; Qin, Xiao ³ ; de Beeck, Michiel Op ⁴ ; He, Conglian ¹ ; Jian, Li ⁵ ; Chen, Yi ¹ ;

作者机构： 1.Yunnan Acad Tobacco Agr Sci, Agron Ctr, Kunming, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming, Yunnan, Peoples R China

3.Hunan Zhangjiajie Municipal Tobacco Co, Raw Mat Ctr, Zhangjiajie, Hunan, Peoples R China

4.Lund Univ, Ctr Environm & Climate Res, Lund, Sweden

5.Chinense Acad Sci, Key Lab Urban Environm & Hlth, Inst Urban Enviroment, Xiamen, Fujian, Peoples R China

关键词： tobacco; proteomics; leaves and roots; plant hormones; topping

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Introduction Until now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways.Methods This study examines the regulatory mechanisms of hormones in the roots and leaves of tobacco plants with and without topping at the protein level.Results The results demonstrate that, compared with non-topped plants, topping leads to a decrease in the levels of IAA (auxin), ABA (abscisic acid), and GA (gibberellin) hormones in the leaves, whereas the content of the JA (jasmonic acid) hormone increases. Furthermore, in the roots, topping results in an increase in the levels of IAA, ABA, and JA hormones, along with a decrease in GA content. In the leaves, a total of 258 significantly different proteins were identified before and after topping, with 128 proteins upregulated and 130 proteins downregulated. In the roots, there were 439 proteins with significantly different quantities before and after topping, consisting of 211 upregulated proteins and 228 downregulated proteins. Notably, these proteins were closely associated with the metabolic and biosynthetic pathways of secondary metabolites, as indicated by functional categorization.Conclusions When integrating the hormone changes and the proteomics results, it is evident that topping leads to increased metabolic activity and enhanced hormone synthesis in the root system. This research provides a theoretical foundation for further investigations into the regulation and signaling mechanisms of hormones at the protein level before and after topping in plants.