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Adaption of tobacco rattle virus to its solanaceous hosts is related to the codon usage bias of the hosts and that of the viral 16 K gene

文献类型： 外文期刊

作者： Lin, Qi 1 ; Zheng, Yuanting 2 ; Yin, Yueyan 3 ; Chen, Yi 1 ; Li, Miaomiao 1 ; Zheng, Hongying 1 ; Chen, Jianping 1 ; Yan, Fei 1 ; Lu, Yuwen 1 ; Xu, Shengchun 2 ;

作者机构： 1.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

2.Zhejiang Acad Agr Sci, Cent Lab, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Kunming 650223, Peoples R China

关键词： Tobacco rattle virus (TRV); Codon usage bias; Host adaptation; 16K

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）

ISSN： 0929-1873

年卷期： 2023 年 166 卷 1 期

页码：

收录情况： SCI

摘要： Tobacco rattle virus (TRV) is a member of the genus Tobravirus, family Virgaviridae, that infects many different plants from 50 families. TRV-based VIGS vectors are widely used in research into the interaction between viruses and their hosts in numerous plant species, especially those in the family Solanaceae. However, there is little known about the codon bias of solanaceous plants and that of TRV. In a comprehensive codon bias analysis of three plants (Capsicum annuum, Solanum lycopersicum and S. tuberosum), their optimal codons were identified and codon usage bias was shown to result from natural selection / mutation. Comparisons of relative codon deoptimization index (RCDI) and codon adaptation index (CAI) of TRV genes suggested that TRV may be better adapted to C. annuum than to S. lycopersicum and S. tuberosum. The average CAI of the 16 K gene, which is the RNA silencing suppressor of TRV, was higher than that of the other three genes in all three hosts, suggesting that the 16 K might be important for viral adaption to different solanaceous hosts. In a transient expression assay in leaves of Nicotiana benthamiana, a GFP-tagged clone of the TRV 16 K with deoptimized codon was expressed significantly less than a wild type sequence of TRV 16 K providing evidence that the virus-encoding silencing suppressor is significantly adapted to the codon usage bias of its hosts.

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