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Prauserella flavalba sp. nov., a novel species of the genus Prauserella, isolated from alkaline soil

文献类型： 外文期刊

作者： Li, Qinyuan 1 ; Ruckert, Christian 2 ; Li, Guiding 1 ; Huang, Ping 3 ; Schneider, Olha 4 ; Kalinowski, Jorn 5 ; Jiang, 1 ;

作者机构： 1.Yunnan Univ, Sch Life Sci, Yunnan Inst Microbiol, Kunming 650091, Yunnan, Peoples R China

2.Univ Bielefeld, Microbial Genom & Biotechnol Ctr Biotechnol CeBiT, Sequenz 1, D-33615 Bielefeld, Germany

3.Yunnan Acad Agr Sci, Inst Food Crop, Kunming 650205, Yunnan, Peoples R China

4.Norwegian Univ Sci & Technol, Dept Biotechnol, N-7491 Trondheim, Norway

5.Univ Vienna, Dept Pharmacognosy, A-1090 Vienna, Austria

关键词： Prauserella flavalba sp. nov.; polyhasic taxonomy; 16S rRNA gene; secondary metabolite biosynthesis gene clusters

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 1 期

页码：

收录情况： SCI

摘要： A Gram-strain positive, mycelium-forming actinomycete, YIM 121212(T), was isolated from an alkaline soil sample collected in Yunnan province, PR China. Classification using a polyphasic approach indicated that YIM 121212(T) represents a member of the genus Prauserella, and is closely related to Prauserella coralliicola SCSIO 11529(T) (99.31 %), Prauserella endophytica SP28S3(T) (99.17 %), Prauserella soli 12-833(T) (97.43 %), Prauserella oleivorans RIPIT (97.03 %), Prauserella marina MS498(T) (96.74 %), Prauserella rugosa DSM 43194(T) (96.54 %) and Prauserella muralis 05-Be-005(T) (95.92 %). Average nucleotide identity values (ANI) of YIM 121212(T) to P. coralliicola DSM 45821(T) and P. endophytica CGMCC 4.7182(T) were 93.1 and 92.8 %, respectively, which were lower than the threshold of 95 %. The digital DNA-DNA hybridization (dDDH) values between YIM 121212(T) and these two species were 50.8 and 49.9%, respectively and thus were also well below the cut off value (>70%) for species delineation. The DNA G+C content of YIM 121212(T) is 70.8 mol%. Major fatty acids are iso-C-16:0, iso-C16:1H, C-16:1 omega 7c/iso-C-15:0 2OH, C-17:1 omega 6c, and C-17:1 omega 8c. The predominant menaquinone is MK-9(H-4). The polar lipid profile consists of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmethylethanolamine (PME), phosphatidylinositol (PI), and phosphatidylinositol mannoside (PIM). The draft genomes were further analyzed for the presence of secondary metabolite biosynthesis (SMB) gene clusters. On the basis of the above observations, YIM121212(T) can be distinguished from closely related species belonging to the genus Prauserella. Thus, YIM121212(T) represents a novel species of the genus Prauserella, for which the name Prauserella flavalba sp. nov. is proposed. The type strain is YIM121212(T) (=CCTCC AA 2013011(T)=DSM 45973(T)).

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