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Transcriptome Analysis of the Salt-Treated Actinidia deliciosa (A. Chev.) C. F. Liang and A. R. Ferguson Plantlets

文献类型： 外文期刊

作者： Wu, Jiexin 1 ; Wei, Zhuo 1 ; Zhao, Wenjuan 1 ; Zhang, Zhiming 1 ; Chen, Daming 2 ; Zhang, Hanyao 1 ; Liu, Xiaozhen 2 ;

作者机构： 1.Southwest Forestry Univ, Key Lab Forest Resources Conservat & Utilizat Sout, Minist Educ, Kunming 650224, Peoples R China

2.Yunnan Acad Agr Sci, Res Inst Agr Ecol Hot Areas, Yuanmou 651300, Peoples R China

关键词： Actinidia deliciosa; salt treatment; transcriptome analysis; RT-qPCR

期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （ 影响因子：3.1； 五年影响因子：3.3 ）

ISSN： 1467-3037

年卷期： 2023 年 45 卷 5 期

页码：

收录情况： SCI

摘要： The area of saline land in the world is quite large, and there is broad room for its development and usage. 'Xuxiang' is an Actinidia deliciosa variety that is tolerant to salt and can be planted in an area of light-saline land, and has good comprehensive characteristics and high economic value. However, the molecular mechanism of salt tolerance is unknown at present. To understand the molecular mechanism of salt tolerance, the leaves of A. deliciosa 'Xuxiang' were used as explants to establish a sterile tissue culture system, and plantlets were obtained using this system. One percent concentration (w/v) of sodium chloride (NaCl) was employed to treat the young plantlets cultured in Murashige and Skoog (MS) medium, then RNA-seq was used for transcriptome analysis. The results showed that the genes related to salt stress in the phenylpropanoid biosynthesis pathway and the anabolism of trehalose and maltose pathways were up-regulated; however, those genes in the plant hormone signal transduction and metabolic pathways of starch, sucrose, glucose, and fructose were down-regulated after salt treatment. The expression levels of ten genes that were up-regulated and down-regulated in these pathways were confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. The salt tolerance of A. deliciosa might be related to the expression level changes in the genes in the pathways of plant hormone signal transduction, phenylpropanoid biosynthesis, and starch, sucrose, glucose, and fructose metabolism. The increased expression levels of the genes encoding alpha-trehalose-phosphate synthase, trehalose-phosphatase, alpha-amylase, beta-amylase, feruloyl-CoA 6-hydroxylase, ferulate 5-hydroxylase, and coniferyl-alcohol glucosyl transferase might be vital to the salt stress response of the young A. deliciosa plants.

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