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In vitro PROPAGATION OF Liparis nervosa (Thunb.) Lindl., AN ENDANGERED MEDICINAL ORCHID

文献类型： 外文期刊

作者： Ren, Yan 1 ; Gao, Jin-Rong 1 ; Cai, Shou-Meng 1 ; Yan, Hong-Mei 2 ; Huang, Heng-Yu 1 ;

作者机构： 1.Yunnan Univ Chinese Med, Yunnan Breeding & Cultivat Res & Dev Ctr Endangere, 1076 Yuhua Rd, Kunming 650500, Peoples R China

2.Yunnan Acad Agr Sci, Inst Qual Stand & Testing Technol, Kunming 650223, Peoples R China

关键词： Key words: in vitro regeneration; proliferation method; basal stem cluster bud; tiller bud; protocorm-like; body; Liparis nervosa (Thunb.) Lindl.

期刊名称：ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS （ 影响因子：0.7； 五年影响因子：1.0 ）

ISSN： 1644-0692

年卷期： 2023 年 22 卷 5 期

页码：

收录情况： SCI

摘要： In vitro regeneration was studied to protect the rare Chinese medicinal orchid Liparis nervosa (Thunb.) Lindl. The mixtures of protocorm and seeding and the stem tip were used as explants. The results revealed that the best essential medium for L. nervosa growth was 1/3 MS medium with 25 g L-1 sucrose, 50 g L-1 banana puree, 40 g L-1 mashed potato, and 1.0 g L-1 AC (MS1); MS1 medium with 0.5 mg L-1 BA, 0.05 mg L-1 2,4-D, and 1.5 mg L-1 NAA was optimal for proliferation. When stem tips were cultured in a proliferation medium, four types of proliferation occurred: basal stem cluster bud (occurring at the basal node), tiller bud (occurring at the root), protocorm-like body (occurring at the plant's base incision), and high-position bud (occurring on plant stem nodes other than the basal nodes). Four methods produced 10.12 proliferation co -efficients. In the MS1 medium with 0.5 mg L-1 NAA, the plantlets rooted 100%, and the rooted plantlets survived 100% after domestication and transplantation.

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