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Development of InDel Markers for Gypsophila paniculata Based on Genome Resequencing

文献类型： 外文期刊

作者： Jin, Chunlian 1 ; Bin Liu 2 ; Ruan, Jiwei 1 ; Yang, Chunmei 1 ; Li, Fan 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Natl Engn Res Ctr Ornamental Hort, Floriculture Res Inst, Key Lab Flower Breeding Yunnan Prov, Kunming 650205, Yunnan, Peoples R China

2.Yunnan Univ, Sch Agr, Kunming 650500, Yunnan, Peoples R China

关键词： Gypsophila paniculata; InDel marker; genetic map; polymorphism

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

ISSN：

年卷期： 2022 年 8 卷 10 期

页码：

收录情况： SCI

摘要： Gypsophila paniculata is the only species in the genus Gypsophila that has been used as cut flowers, and the sequencing of its genome has just been completed, opening a new chapter in its molecular genetic breeding. The molecular marker system is the basis for genetic molecular research in the era of genomics, whereas it is still a gap for G. paniculata. In this study, we constructed a genome-wide InDel marker system of G. paniculata after genome resequencing of another wild-type accession with white flowers. Consequently, 407 InDel markers at a distance of similar to 2 Mb were designed for all 17 chromosomes. Later, the validation of these markers by PCR revealed that 289 markers could distinguish alleles of the two wild-type alleles clearly. The predicted polymorphisms of two wild-type alleles were then transferred to the commercial cultivars, which displayed a rich polymorphism among four commercial cultivars. Our research established the first genome-level genetic map in G. paniculata, providing a comprehensive set of marker systems for its molecular research.

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