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Molecular Characterization and Gene Expression of Trehalase in the Bumblebee, Bombus lantschouensis (Hymenoptera: Apidae)

文献类型： 外文期刊

作者： Qin, Jiamin 1 ; Liu, Feng 3 ; Wu, Jie 1 ; He, Shaoyu 4 ; Imran, Muhammad 5 ; Lou, Wen 3 ; Li-Byarlay, Hongmei 6 ; Luo, Shudong 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Apicultural Res, Key Lab Pollinating Insect Biol, Minist Agr & Rural Affairs, Beijing, Peoples R China

2.Yunnan Acad Agr Sci, Sericulture & Apiculture Res Inst, Mengzi, Peoples R China

3.Apiculture Inst Jiangxi Prov, Nanchang, Jiangxi, Peoples R China

4.Yunnan Agr Univ, Eastern Bee Res Inst, Kunming, Yunnan, Peoples R China

5.Univ Poonch Rawalakot, Dept Entomol, Jammu, Jammu & Kashmir, India

6.Cent State Univ, Dept Agr & Life Sci, Agr Res & Dev Program, Wilberforce, OH USA

关键词： Bombus lantschouensis; trehalase; sequence analysis; stress conditions; gene expression

期刊名称：SOCIOBIOLOGY （ 影响因子：0.864； 五年影响因子：1.0 ）

ISSN： 0361-6525

年卷期： 2021 年 68 卷 4 期

页码：

收录情况： SCI

摘要： Trehalose provides the main energy source for the physiological activities of insects, especially in adverse conditions. Trehalase is the only enzyme that hydrolyzes trehalose, therefore it is important to clarify the distribution and expression of trehalase under adverse conditions such as high temperatures and starvation. Here, we have cloned the trehalase genes and investigated their expression in different tissues, at multiple development stages, and with the treatments of high temperature and starvation in Bombus lantschouensis, which is considered to be one of the most commercially viable native species in China. The results suggest that the membrane-bound (BlTre-2) cDNA has an open reading frame of 1986 nucleotides, which encodes a protein of 662 amino acids, and two putative transmembrane domains. qRT-PCR analysis indicated that BlTre-2 was expressed in 10 tissues and at nine development stages, with the highest expression in general in 30-day-old workers, and in ovarian tissue in particular. The expression of BlTre-1 for 15-day-old workers which were exposed to a pre-treatment of 45 degrees C increased over the first 5 h and subsequently decreased over time. In contrast the expression of BITre-2 consistently decreased over time. The highest expression levels of BlTre-1 and BlTre-2 were observed the newly emerged adult workers when starved for 16-20 h. These results indicate that BlTre-2 may be part of the carbohydrate metabolism of the bumblebee, and that BlTre-1 is a key gene regulating energy metabolism and providing trehalose when exposed to a high temperature. Both BlTre-1 and BlTre-2 may balance trehalose and provide energy when B. lantschouensis is starved.

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