您好，欢迎访问云南省农业科学院 机构知识库！

Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers

文献类型： 外文期刊

作者： Yan, Huijun 1 ; Shi, Shaochuan 2 ; Ma, Nan 2 ; Cao, Xiaoqian 2 ; Zhang, Hao 1 ; Qiu, Xianqin 1 ; Wang, Qigang 1 ; Jian, H 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China

2.China Agr Univ, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Cro, Beijing 100193, Peoples R China

3.China Agr Univ, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Cro, Beijing 100193,

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2018 年 60 卷 1 期

页码：

收录情况： SCI

摘要： Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors. Virus-induced gene silencing (VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose, called graft-accelerated VIGS', where axillary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR1, RhAG, and RhNUDX1 in rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台