文献类型： 外文期刊

作者： Xia, Chaoshui ¹ ; Cao, Yiyang ¹ ; Gan, Weixin ¹ ; Lin, Huifeng ¹ ; Li, Huayang ⁴ ; Lin, Fazhuang ¹ ; Lu, Zhenhong ⁵ ; Chen, Weiting ¹ ;

作者机构： 1.Sanming Acad Agr Sci, Sanming 365509, Peoples R China

2.Fujian Key Lab Crop Genet Improvement & Innovat Ut, Sanming 365509, Peoples R China

3.Fujian Agr & Forestry Univ, Hort Coll, Fuzhou 350002, Peoples R China

4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

5.Yunnan Acad Agr Sci, Kunming 650205, Peoples R China

关键词： Gerbera hybrida; preservation; response surface; antioxidant activity; transcriptome

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN：

年卷期： 2025 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Gerbera (Gerbera hybrida) is a popular cut flower on the market, so extending its vase life (VL) is an important goal in the horticultural industry. The aim of this study was to improve the freshness of gerbera cut flowers through the optimal solution (OS) and to analyze its preservation mechanism. We used chitosan (COS), calcium chloride (CaCl2), and citric acid (CA) as the main ingredients of the vase solution and determined the OS ratio of 104 mg/L of COS, 92 mg/L of CA, and 93 mg/L of CaCl2 using the Box-Behnken design-response surface method (BBD-RSM). Gerbera preservation results showed that the VL of the OS was 14.5 days, which was significantly longer than that of flowers maintained in the Basic Vase Solution (BVS) and the Commercial Formulation (CF) and was highly consistent with the theoretical VL of 14.57 d. Transcriptome analysis indicated that the OS might extend VL by regulating phytohormone signaling pathways, such as cytokinin and salicylic acid signaling. The qRT-PCR analysis of key candidate genes supported these findings, with significant upregulation observed in genes related to cytokinin synthesis (e.g., GhIPT1 and GhIPT9), salicylic acid signaling related to pathogen defense (e.g., GhTGA1, GhTGA4, GhNPR1, and GhRBOHA), and plant wax synthesis and stress response (e.g., GhKCS5, GhCUT1, and GhKCS6). Further, transcriptome GO-enrichment and physiological analysis showed that the OS might extend VL of Gerbera cut flowers by scavenging reactive oxygen species, including by activating the expression of genes related to oxidoreductase activity and the activities of antioxidant-system-related enzymes catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and superoxide dismutase (SOD), while decreasing the malondialdehyde (MDA) content. These results provide valuable insights into the mechanisms underlying the extended VL of gerbera cut flowers and offer a foundation for developing more effective preservation techniques.