文献类型： 外文期刊

作者： Kim, Sun Ha ¹ ; Ji, Shi-Dong ¹ ; Lee, Hyun-Sook ¹ ; Jeon, Yun-A ¹ ; Shim, Kyu-Chan ¹ ; Adeva, Cheryl ¹ ; Luong, Ngoc Ha ¹ ; Yuan, Pingrong ¹ ; Kim, Hyun-Jung ² ; Tai, Thomas H. ³ ; Ahn, Sang-Nag ¹ ;

作者机构： 1.Chungnam Natl Univ, Coll Agr & Life Sci, Dept Agron, Daejeon, South Korea

2.LG Chem Ltd, Seoul, South Korea

3.USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA

4.Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

5.Nanyang Normal Univ, Sch Life Sci & Agr Engn, Nanyang City, Peoples R China

6.Natl Inst Crop Sci, Crop Breeding Div, Wanju Gun, South Korea

7.Yunnan Acad Agr Sci, Inst Food Crop Res, Kunming, Yunnan, Peoples R China

关键词： hybrid weakness; dark tip embryo; rice; floral organ determination; seed germination; abscisic acid

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 12 卷

页码：

收录情况： SCI

摘要： A novel hybrid weakness gene, DTE9, associated with a dark tip embryo (DTE) trait, was observed in CR6078, an introgression line derived from a cross between the Oryza sativa spp. japonica "Hwayeong" (HY) and the wild relative Oryza rufipogon. CR6078 seeds exhibit protruding embryos and flowers have altered inner floral organs. DTE9 was also associated with several hybrid weakness symptoms including decreased grain weight. Map-based cloning and transgenic approaches revealed that DTE9 is an allele of OsMADS8, a MADS-domain transcription factor. Genetic analysis indicated that two recessive complementary genes were responsible for the expression of the DTE trait. No sequence differences were observed between the two parental lines in the OsMADS8 coding region; however, numerous single nucleotide polymorphisms were detected in the promoter and intronic regions. We generated overexpression (OX) and RNA interference (RNAi) transgenic lines of OsMADS8 in HY and CR6078, respectively. The OsMADS8-OX lines showed the dark tip embryo phenotype, whereas OsMADS8-RNAi recovered the normal embryo phenotype. Changes in gene expression, including of ABCDE floral homeotic genes, were observed in the OsMADS8-OX and OsMADS8-RNAi lines. Overexpression of OsMADS8 led to decreased expression of OsEMF2b and ABA signaling-related genes including OsVP1/ABI3. HY seeds showed higher ABA content than CR6078 seeds, consistent with OsMADS8/DTE9 regulating the expression of genes related ABA catabolism in CR6078. Our results suggest that OsMADS8 is critical for floral organ determination and seed germination and that these effects are the result of regulation of the expression of OsEMF2b and its role in ABA signaling and catabolism.