文献类型： 外文期刊

作者： Zhang, Jiahui ¹ ; Zhang, Wenhao ³ ; Wei, Lin ¹ ; Zhang, Lidan ¹ ; Liu, Jiali ¹ ; Huang, Shumin ¹ ; Li, Sheng ¹ ; Yang, Weike ⁴ ; Li, Kang ¹ ;

作者机构： 1.South China Normal Univ, Sch Life Sci, Inst Insect Sci & Technol, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Guangzhou 510631, Peoples R China

2.South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

3.Huaihua Univ, Coll Biol & Food Engn, Huaihua 418000, Peoples R China

4.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi 661100, Yunnan, Peoples R China

5.South China Normal Univ, Guangmeiyuan R&D Ctr, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Meizhou 514779, Peoples R China

关键词： apoptosis; Drosophila; E93; RHG genes; salivary gland; transcription

期刊名称：INSECT SCIENCE （ 影响因子：3.605； 五年影响因子：3.323 ）

ISSN： 1672-9609

年卷期：

页码：

收录情况： SCI

摘要： 20-hydroxyecdysone (20E) induced transcription factor E93 is important for larval-adult transition, which functions in programmed cell death of larval obsolete tissues, and the formation of adult new tissues. However, the apoptosis-related genes directly regulated by E93 are still ambiguous. In this study, an E93 mutation fly strain was obtained by clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9-mediated long exon deletion to investigate whether and how E93 induces apoptosis during larval tissues metamorphosis. The transcriptional profile of E93 was consistent with 3 RHG (rpr, hid, and grim) genes and the effector caspase gene drice, and all their expressions peaked at the initiation of apoptosis during the degradation of salivary glands. The transcription expression of 3 RHG genes decreased and apoptosis was blocked in E93 mutation salivary gland during metamorphosis. In contrast, E93 overexpression promoted the transcription of 3 RHG genes, and induced advanced apoptosis in the salivary gland. Moreover, E93 not only enhance the promoter activities of the 3 RHG genes in Drosophila Kc cells in vitro, but also in the salivary gland in vivo. Our results demonstrated that 20E induced E93 promotes the transcription of RHG genes to trigger apoptosis during obsolete tissues degradation at metamorphosis in Drosophila.