An atypical NLR pair TdCNL1/TdCNL5 from wild emmer confers powdery mildew resistance in wheat
文献类型： 外文期刊
作者： Zhu, Keyu 1 ; Li, Miaomiao 1 ; Dong, Lingli 1 ; Zhang, Huaizhi 1 ; Zhang, Deyun 2 ; Lu, Ping 1 ; Wu, Qiuhong 3 ; Xie, Jingzhong 1 ; Chen, Yongxing 3 ; Guo, Guanghao 1 ; Zhang, Panpan 4 ; Li, Beibei 3 ; Li, Wenling 3 ; Dong, Lei 3 ; Hou, Yikun 1 ; Yang, Yijun 1 ; Qiu, Dan 1 ; Wang, Gaojie 1 ; Huang, Baoge 1 ; Cui, Xuejia 1 ; Fu, Hongkui 1 ; Yuan, Chengguo 6 ; Fahima, Tzion 7 ; Nevo, Eviatar 7 ; Li, Hongjie 3 ; Rong, Junkang 8 ; Hua, Wei 3 ; Liu, Zhiyong 1 ;
作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Seed Innovat, Beijing, Peoples R China
2.Chaozhou Hybribio Biochem Ltd, Chaozhou, Peoples R China
3.Inst Biotechnol, Xianghu Lab, Hangzhou, Peoples R China
4.Yunnan Acad Agr Sci, Tea Res Inst, Kunming, Peoples R China
5.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
6.Hebei Gaoyi Stock Seed Farm, Gaoyi, Peoples R China
7.Univ Haifa, Inst Evolut, Haifa, Israel
8.Zhejiang Agr & Forestry Univ, Coll Adv Agr Sci, Linan, Peoples R China
9.Zhejiang Acad Agr Sci, Hangzhou, Peoples R China
10.Hainan Seed Ind Lab, Sanya, Peoples R China
期刊名称：NATURE GENETICS （ 影响因子：29.0； 五年影响因子：37.4 ）
ISSN： 1061-4036
年卷期： 2025 年 57 卷 6 期
页码：
收录情况： SCI
摘要： Resistance to wheat powdery mildew is commonly mediated by individual resistance proteins, most of which encode nucleotide-binding leucine-rich repeat (NLR) receptors. Here we report that the powdery mildew resistance gene MLIW170/PM26 in wild emmer and bread wheat derivatives is determined by a genetically linked atypical NLR pair TdCNL1/TdCNL5. Map-based cloning and PacBio HiFi long-read sequencing revealed that TdCNL1 encodes an atypical coiled-coil-domain-containing NLR protein (CNL) fused with a new potassium-dependent sodium-calcium exchanger integrated domain, whereas TdCNL5 encodes a canonical CNL protein. Mutagenesis and virus-induced gene silencing experiments indicated that both TdCNL1 and TdCNL5 are essential for powdery mildew resistance. Transgenic plants with TdCNL1 alone or TdCNL1/TdCNL5 together show resistance, whereas Fielder with TdCNL5 alone was susceptible. Geographically, MLIW170/PM26 occurs in a few Southern populations of wild emmer wheat. Our study highlights an atypical NLR pair coordinately regulating powdery mildew resistance and provides a diversified resistance gene resource for wheat improvement.
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