Optimization of ultrasonic extraction by response surface methodology combined with ultrafast liquid chromatography-ultraviolet method for determination of four iridoids in Gentiana rigescens
文献类型： 外文期刊
作者： Pan, Yu 1 ; Zhang, Ji 1 ; Shen, Tao 3 ; Zuo, Zhi-Tian 1 ; Jin, Hang 1 ; Wang, Yuan-Zhong 1 ; Li, Wan-Yi 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Peoples R China
2.Yunnan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Kunming 650500, Peoples R China
3.Yuxi Normal Univ, Coll Resources & Environm, Yuxi 653100, Peoples R China
关键词： Gentiana rigescens;iridoid glycosides;response surface methodology;ultrafast liquid chromatography-ultraviolet;ultrasonic assisted extraction
期刊名称：JOURNAL OF FOOD AND DRUG ANALYSIS （ 影响因子：6.079； 五年影响因子：6.36 ）
ISSN： 1021-9498
年卷期： 2015 年 23 卷 3 期
页码：
收录情况： SCI
摘要： Gentiana rigescens is a rich source of iridoids and is commonly used as a folk medicine for treatment of hepatitis and cholecystitis for over 1000 years. A rapid ultrafast liquid chromatography ultraviolet method was developed for simultaneous determination of four major iridoid glycosides in G. rigescens. Response surface methodology based. on the Box -Behnken design was applied to optimize the extraction conditions of iridoid glycosides. Using the Shim-Pack XR-ODS III, four iridoid glycosides were efficiently separated with an acetonitrile:0.1% formic acid aqueous solution gradient at a flow rate of 0.25 mL/min for 8 minutes. All the regression equations revealed a good linear relationship (R-2 > 0.9995). The intraday and interday variations were <1.95%. The recoveries ranged from 99.7% to 103.2%. The optimal extraction conditions were as follows: methanol concentration, 82%; the ratio of liquid to solid material, 68:1 (mL/g); and extraction time, 32 minutes. The yield of the four iridoid glycosides under the optimal process was found to be 63.08 mg/g, which was consistent with the predicted yield. In addition, the total content of 50 cultivated samples from Lincang, Yunnan, China, was within the range of 33.6-113.26 mg/g, which provides a more reasonable foundation for utilization of G. rigescens. Copyright (c) 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Based on metabolomics and fourier transforms near infrared spectroscopy characterization of Lanxangia tsaoko chemical profile differences among fruit types and development of rapid identification and nutrient prediction models
作者：Fu, Deng-Ke;Yang, Wei-Ze;Yang, Mei-Quan;Yang, Tian-Mei;Wang, Yuan-Zhong;Zhang, Jin-Yu;Fu, Deng-Ke
关键词：Lanxangia tsao-ko; Phenotype; Metabolic differences; 2DCOS; ResNet; PLSR
-
FT-NIR Spectra of Different Dimensions Combined with Machine Learning and Image Recognition for Origin Identification: An Example of Panax notoginseng
作者：Zuo, Zhi-Tian;Yao, Zeng-Yu;Zuo, Zhi-Tian;Wang, Yuan-Zhong
关键词：
-
Application of ATR-FTIR Spectrum Combined With Ensemble Learning and Deep Learning for Identification of Amomum tsao-ko at Different Drying Temperatures
作者：He, Gang;Yang, Shao-bing;Wang, Yuan-zhong;He, Gang
关键词：Amomum tsao-ko; deep learning; drying temperatures; ensemble learning; machine learning
-
Spatial and temporal distribution characteristics of Paris polyphylla var. yunnanensis and the prediction of steroidal saponins content
作者：Zhong, Chen;Li, Li;Zhong, Chen;Wang, Yuan-Zhong
关键词：Paris polyphylla var. yunnanensis; Habitat suitability; FT-IR spectroscopy; Chemometrics; Steroidal saponins
-
Anti-Inflammatory Dimeric and Trimeric Flavonoids from the Roots ofPistacia weinmannifolia
作者：Lee, Seong Mi;Ryu, Hyung Won;Kim, Hyoung-Geun;Jo, Yang Hee;Lee, Su Ui;Oh, Eun Sol;Oh, Sei-Ryang;Lee, Seong Mi;Ryu, Hyung Won;Kim, Hyoung-Geun;Jo, Yang Hee;Lee, Su Ui;Oh, Eun Sol;Oh, Sei-Ryang;Lee, Seong Mi;Hwang, Bang Yeon;Lee, Seong Mi;Park, Kyoung Jin;Lee, Sang Woo;Choi, Sangho;Li, Wan-Yi
关键词：
-
Distinct Nitrogen Forms Shape Flavonoid Biosynthesis and Gene-Metabolite Networks in Erigeron breviscapus
作者：Yang, Yan;Li, Yongmei;Yang, Yan;Li, Linyu;Wang, Xing;Yang, Bin;Ma, Weisi;Jin, Hang
关键词：nitrogen forms; flavonoid biosynthesis; erigeron breviscapus; gene-metabolite networks
-
A Comprehensive Quality Evaluation for Gentiana Rigescens Franch. by Fingerprinting Combined with Chemometrics and Network Pharmacology
作者：Li, Chaoping;Zhu, Xinyan;Zhang, Rongping;Li, Chaoping;Zhu, Xinyan;Zhang, Rongping;Li, Chaoping;Wang, Yuanzhong;Shen, Tao
关键词：
Gentiana rigescens Franch.; Multidimensional spectral images; Chemometrics; Quality differences; Quality evaluation
