您好，欢迎访问云南省农业科学院 机构知识库！

Identification of a new tospovirus causing necrotic ringspot on tomato in China

文献类型： 外文期刊

作者： Yin, Yueyan 1 ; Zheng, Kuanyu 1 ; Dong, Jiahong 1 ; Fang, Qi 1 ; Wu, Shiping 3 ; Wang, Lishuang; Zhang, Zhongkai 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Key Lab Southwestern Crop Gene Resources & Germpl, Yunnan Key Lab Agr Biotechnol,Minist Agr, Kunming 650223, Peoples R China

2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Peoples R China

3.Guizhou Acad Ag

关键词： Tospovirus;Tomato necrotic spot virus;S RNA

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2014 年 11 卷

页码：

收录情况： SCI

摘要： Background: Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. So far, eight tospoviruses were reported in China. Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China. Finding: ELISA experiments showed that crude saps of the diseased tomato fruit samples reacted with antiserum against Tomato zonate spot virus (TZSV). Electron microscopy detected presence of quasi-spherical, enveloped particles of 80-100 nm in such saps. The putative virus isolate was designated 2009-GZT. Mechanical back-inoculation showed that 2009-GZT could infect systemically some solanaceous crop and non-crop plants including Capiscum annuum, Datura stramonium, Nicotiana benthamiana, N. rustica, N. tabacum and Solanum lycopersicum. The 3012 nt full-length sequence of 2009-GZT S RNA shared 68.2% nt identity with that of Calla lily chlorotic spot virus (CCSV), the highest among all compared viruses. This RNA was predicted to encode a non-structural protein (NSs) (459 aa, 51.7 kDa) and a nucleocapsid protein (N) (278 aa, 30.3 kDa). The N protein shared 85.8% amino acid identity with that of CCSV. The NSs protein shared 82.7% amino acid identity with that of Tomato zonate spot virus(TZSV). Conclusion: Our results indicate that the isolate 2009-GZT is a new species of Tospovirus, which is named Tomato necrotic spot virus (TNSV). This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.

  • 相关文献

[1]A new tospovirus causing chlorotic ringspot on Hippeastrum sp in China. Dong, J. H.,Yin, Y. Y.,Fang, Q.,Zhang, Z. K.,Mcbeath, J. H.. 2013

[2]云南西瓜银灰斑驳病毒病害的鉴定. 尹跃艳,卢训,李婷婷,李红祥,郭淼,赵丽玲,张晓云,丁铭. 2016

[3]Verification of serological relationship between two phylogenetically related peanut-infecting Tospovirus species. Kang, Ya-Chi,Liao, Chih-Hsuan,Chou, Wan-Chen,Chen, Tsung-Chi,Kang, Ya-Chi,Yeh, Shyi-Dong,Liu, Fang-Lin,Yeh, Shyi-Dong,Dong, Jia-Hong,Chen, Tsung-Chi.

[4]Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China. Chen, Yu-Han,Chien, Wan-Chu,Sun, Jing-Hua,Wang, Yun-Chi,Chen, Tsung-Chi,Dong, Jiahong,Zheng, Kuanyu,Wu, Kuo,Yeh, Shyi-Dong,Yeh, Shyi-Dong,Chen, Tsung-Chi. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台