文献类型： 外文期刊
作者： Yin, Yueyan 1 ; Zheng, Kuanyu 1 ; Dong, Jiahong 1 ; Fang, Qi 1 ; Wu, Shiping 3 ; Wang, Lishuang; Zhang, Zhongkai 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Key Lab Southwestern Crop Gene Resources & Germpl, Yunnan Key Lab Agr Biotechnol,Minist Agr, Kunming 650223, Peoples R China
2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Peoples R China
3.Guizhou Acad Ag
关键词： Tospovirus;Tomato necrotic spot virus;S RNA
期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）
ISSN： 1743-422X
年卷期： 2014 年 11 卷
页码：
收录情况： SCI
摘要： Background: Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. So far, eight tospoviruses were reported in China. Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China. Finding: ELISA experiments showed that crude saps of the diseased tomato fruit samples reacted with antiserum against Tomato zonate spot virus (TZSV). Electron microscopy detected presence of quasi-spherical, enveloped particles of 80-100 nm in such saps. The putative virus isolate was designated 2009-GZT. Mechanical back-inoculation showed that 2009-GZT could infect systemically some solanaceous crop and non-crop plants including Capiscum annuum, Datura stramonium, Nicotiana benthamiana, N. rustica, N. tabacum and Solanum lycopersicum. The 3012 nt full-length sequence of 2009-GZT S RNA shared 68.2% nt identity with that of Calla lily chlorotic spot virus (CCSV), the highest among all compared viruses. This RNA was predicted to encode a non-structural protein (NSs) (459 aa, 51.7 kDa) and a nucleocapsid protein (N) (278 aa, 30.3 kDa). The N protein shared 85.8% amino acid identity with that of CCSV. The NSs protein shared 82.7% amino acid identity with that of Tomato zonate spot virus(TZSV). Conclusion: Our results indicate that the isolate 2009-GZT is a new species of Tospovirus, which is named Tomato necrotic spot virus (TNSV). This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.
- 相关文献
作者其他论文 更多>>
-
Adoption of the 2A Ribosomal Skip Principle to Track Assembled Virions of Pepper Mild Mottle Virus in Nicotiana benthamiana
作者：Jiao, Mengting;Tian, Yanzhen;Lei, Jianing;Lin, Lin;Wu, Jian;Lu, Yuwen;Zheng, Hongying;Yan, Fei;Peng, Jiejun;Jiao, Mengting;Tian, Yanzhen;Lei, Jianing;Lin, Lin;Wu, Jian;Lu, Yuwen;Zheng, Hongying;Yan, Fei;Peng, Jiejun;Jiao, Mengting;Wang, Jianguang;Yin, Yueyan
关键词：2A; pepper mild mottle virus; coat protein; self-assembly
-
Complete genome sequence of a novel totivirus isolated from the leaves of Myrica rubra
作者：Luo, Weihao;Zhang, Lei;Dong, Xian;Ji, Pengzhang;Dong, Jiahong;Luo, Weihao;Zhang, Lei;Dong, Xian;Ji, Pengzhang;Dong, Jiahong;Zhang, Lizhen;Wu, Kuo
关键词：
-
Occurrence and characterization of viruses infecting Amorphophallus in Yunnan, China
作者：Dong, Jiahong;Zhu, Ting;Zhang, Boxin;Zhang, Lei;Dong, Jiahong;Zhu, Ting;Zhang, Boxin;Zhang, Lei;Lv, Rui;Xi, Dehui;Dong, Kun;Yin, Jianqing;Lu, Jun;Li, Yu;Zhang, Lizhen;Chen, Yongdui;Wu, Kuo;Yin, Xiangao
关键词：
-
Complete genome sequence of polygonatum kingianum mottle virus infecting Polygonatum kingianum Coll. et Hemsl in Yunnan, China
作者：Wang, Maosen;Su, Xiaoxia;Zhang, Fan;Wang, Tiantian;Zheng, Kuanyu;Zhang, Zhongkai;Wang, Maosen;Zhang, Fan
关键词：
-
First report of tomato spotted wilt virus (TSWV) infection on Polygonatum kingianum in China
作者：Du, Yumeng;Yang, Xuwang;Du, Yumeng;Qiu, Runshuang;Zhang, Shuiying;Zhao, Lihua;Zhang, Zhongkai
关键词：
-
Genomic characterization and seed transmission of a novel unclassified partitivirus infecting Polygonatum kingianum Coll. et Hemsl
作者：Zhang, Fan;Su, Xiaoxia;Zhang, Shaozhi;Wang, Tiantian;Zheng, Xue;Wu, Kuo;Zheng, Kuanyu;Zhang, Zhongkai;Zhang, Fan;Su, Xiaoxia;Zhang, Shaozhi;Wang, Tiantian;Zheng, Xue;Wu, Kuo;Zheng, Kuanyu;Zhang, Zhongkai;Wang, Maosen
关键词：Polygonatum kingianum; Partitiviridae; RdRp; CP; Seed transmission
-
Quantification of plasmodesmata frequency under three-dimensional view using focused ion beam-scanning electron microscopy and image analysis
作者：Deng, Yinlu;Wang, Yaqin;Hong, Jian;Li, Zhenghe;Xie, Li;Deng, Yinlu;Li, Zhenghe;Shang, Weina;Shang, Weina;Zhang, Xiaomin;Hong, Jian;Xie, Li;Guo, Jiansheng;Guo, Jiansheng;Zhang, Zhongkai
关键词：PD quantification; PD frequency; Focused ion beam-scanning electron micro; scopy; Image analysis
