Adoption of the 2A Ribosomal Skip Principle to Track Assembled Virions of Pepper Mild Mottle Virus in Nicotiana benthamiana
文献类型： 外文期刊
作者： Jiao, Mengting 1 ; Yin, Yueyan 4 ; Tian, Yanzhen 1 ; Lei, Jianing 1 ; Lin, Lin 1 ; Wu, Jian 1 ; Lu, Yuwen 1 ; Zheng, Hongying 1 ; Yan, Fei 1 ; Wang, Jianguang 3 ; Peng, Jiejun 1 ;
作者机构： 1.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
2.Ningbo Univ, Inst Plant Virol, Key Lab Biotechnol Plant Protect MARA & Zhejiang P, Ningbo 315211, Peoples R China
3.Yunnan Univ, Biocontrol Engn Res Ctr Crop Dis & Pest Yunnan Pro, Sch Ecol & Environm Sci, Kunming 650091, Peoples R China
4.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Kunming 650223, Peoples R China
关键词： 2A; pepper mild mottle virus; coat protein; self-assembly
期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 7 期
页码：
收录情况： SCI
摘要： The coat protein (CP) is an important structural protein that plays many functional roles during the viral cycle. In this study, the CP of pepper mild mottle virus (PMMoV) was genetically fused to GFP using the foot-and-mouth disease virus peptide 2A linker peptide and the construct (PMMoV-GFP(2A)) was shown to be infectious. The systemic spread of the virus was monitored by its fluorescence in infected plants. Electron microscopy and immunocolloidal gold labelling confirmed that PMMoV-GFP(2A) forms rod-shaped particles on which GFP is displayed. Studies of tissue ultrastructure and virion self-assembly confirmed that PMMoV-GFP(2A) could be used to monitor the real-time dynamic changes of CP location during virus infection. Aggregations of GFP-tagged virions appeared as fluorescent plaques in confocal laser microscopy. Altogether, PMMoV-GFP(2A) is a useful tool for studying the spatial and temporal changes of PMMoV CP during viral infection.
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