文献类型： 外文期刊
作者： Ma, ZG 1 ; Yan, B 2 ; Huang, XQ 2 ; Wang, LX 2 ; Zeng, ZK 2 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Biotechnol, Kunming 650223, Peoples R China
2.Yunnan Acad Agr Sci, Inst Biotechnol, Kunming 650223, Peoples R China; Sichuan Univ, Dept Biol, Chengdu 610064, Peoples R China
关键词： Sophora flavescens;lectin;inhibitory activity;cloning
期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）
ISSN： 0577-7496
年卷期： 2001 年 43 卷 8 期
页码：
收录情况： SCI
摘要： A lectin protein(SFL) with molecular weight about 32 kD which markedly agglutinated rabbit and human red blood cells was purified from the roots of Sophora flavescens Ait. This protein, and apparently inhibited the growth of Fusarium vasinfectum Atk., Gibberella saubinetii (Mont.) Sacc., and Piricularia oryzae Cav. A set of degenerate PCR primer was synthesized according to the N-terminal sequence of the purified protein. The full-length cDNA coding the lectin was cloned by RT-PCR and 5'-RACE and sequenced (GenBank AF285121). The deduced amino acid sequence indicates that a preprotein with 284 amino acid residues is firstly translated and then processed to a mature protein with 254 amino acids. A N-Glycosylation site is the Asn 182 residue.
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