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Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)

文献类型： 外文期刊

作者： Li, Y 1 ; Xi, YW 2 ; Zhang, ZK 2 ; Huang, XQ 2 ;

作者机构： 1.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Peking Yale Joint Ctr Plant Mol Genet & Agrobiotec, Beijing 100871, Peoples R China

2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Peking Yale Joint Ctr Plant Mol Genet & Agrobiotec, Beijing 100871, Peoples R China; NW Univ Xian, Dept Biol Engn, Xian 710069, Peoples R China; Yunnan Acad Agr Sci, Inst Biotechnol, Kunming 650223, Peoples R China

关键词： chalcone synthase A;co-suppression;Petunia hybrida;in situ hybridization

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2001 年 44 卷 6 期

页码：

收录情况： SCI

摘要： Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the a-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.

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